增强人类胚胎干细胞由中国医药扶正化瘀

StephenW

Enhancement of hepatocyte differentiation from human embryonic stem cells by Chinese medicine Fuzhenghuayu

    Jiamei Chen, Wei Gao, Ping Zhou, Xiaocui Ma, Benjamin Tschudy-Seney, Chenghai Liu, Mark A Zern, Ping Liu & Yuyou Duan

    Scientific Reports 6, Article number: 18841 (2016)
    doi:10.1038/srep18841
    Download Citation
        Embryonic stem cells | Stem-cell differentiation

Received:
    27 August 2015
Accepted:
    27 November 2015
Published online:
    06 January 2016

Abstract

Chinese medicine, Fuzhenghuayu (FZHY), appears to prevent fibrosis progression and improve liver function in humans. Here we found that FZHY enhanced hepatocyte differentiation from human embryonic stem cells (hESC). After treatment with FZHY, albumin expression was consistently increased during differentiation and maturation process, and expression of metabolizing enzymes and transporter were also increased. Importantly, expression of mesenchymal cell and cholangiocyte marker was significantly reduced by treatment with FZHY, indicating that one possible mechanism of FZHY’s role is to inhibit the formation of mesenchymal cells and cholangiocytes. Edu-labelled flow cytometric analysis showed that the percentage of the Edu positive cells was increased in the treated cells. These results indicate that the enhanced proliferation involved hepatocytes rather than another cell type. Our investigations further revealed that these enhancements by FZHY are mediated through activation of canonical Wnt and ERK pathways and inhibition of Notch pathway. Thus, FZHY not only promoted hepatocyte differentiation and maturation, but also enhanced hepatocyte proliferation. These results demonstrate that FZHY appears to represent an excellent therapeutic agent for the treatment of liver fibrosis, and that FZHY treatment can enhance our efforts to generate mature hepatocytes with proliferative capacity for cell-based therapeutics and for pharmacological and toxicological studies.http://www.nature.com/articles/srep18841?WT.ec_id=SREP-631-20160112&spMailingID=50442231&spUserID=ODY4OTExODM2MwS2&spJobID=841561407&spReportId=ODQxNTYxNDA3S0

StephenW

增强人类胚胎干细胞由中国医药扶正化瘀佳美陈，高炜，周萍，小翠马，本杰明Tschudy - 塞尼，澄海刘，标志着一个Zern，刘平和裕友段科学肝细胞分化的报告6，商品编号： 18841（2016年）DOI：10.1038 / srep18841下载文献胚胎干细胞|干细胞分化
收稿日期：2015年8月27日
接受日期：2015年11月27日
网上发布：2016年1月6日
抽象
中国医药，扶正化瘀（腥），似乎阻止肝纤维化的进展，改善人类的肝脏功能。在这里，我们发现了从人胚胎干细胞（hESC细胞），该扶正化瘀增强肝细胞分化。与扶正化瘀处理后，白蛋白表达一致地分化和成熟过程中增加，和代谢酶和转运的表达也增加。重要的是，间质细胞和胆管细胞标志物的表达通过治疗显著降低与扶正化瘀，表明扶正化瘀的作用的一种可能的机制是抑制间质细胞和胆管细胞的形成。埃杜标记的流式细胞仪分析显示，埃杜阳性细胞的百分比是在处理过的细胞增加。这些结果表明，增强的增殖肝细胞参与，而不是另一种细胞类型。我们的调查还显示，这些改进被扶正化瘀通过激活经典Wnt和E​​RK途径和抑制Notch途径介导。因此，扶正化瘀不仅促进肝细胞分化和成熟，同时也增强肝细胞增殖。这些结果表明，扶正化瘀似乎代表极好的治疗剂的肝纤维化的治疗，而治疗扶正化瘀可以增强我们的努力，以产生成熟的肝细胞与基于细胞的治疗剂和药理学和毒理学研究增殖能力。

newchinabok

我经常用扶正化瘀胶囊，很好抗肝纤药，古方变化过来的，不知现在生产会不会走样

zgct

感谢！

csfluke

中医的疗效终于得到国际权威杂志肯定