突变在S基因和在慢性乙型肝炎中国患者HBsAg和抗-HBs共存重叠

StephenW

Braz J Infect Dis. 2015 Nov 21. pii: S1413-8670(15)00193-2. doi: 10.1016/j.bjid.2015.08.014. [Epub ahead of print]
Mutations in the S gene and in the overlapping reverse transcriptase region in chronic hepatitis B Chinese patients with coexistence of HBsAg and anti-HBs.Ding F1, Miao XL2, Li YX1, Dai JF1, Yu HG3.
Author information

• 1Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, PR China.
• 2Department of Clinical Laboratory, Puai Hospital Affiliated to Tongji Medical College of Huazhong University of Science & Technology, Wuhan 430060, Hubei Province, PR China.
• 3Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, PR China; Institute for Gastroenterology and Hepatology, Wuhan University Medical School, Wuhan 430060, Hubei Province, PR China. Electronic address: [email protected].
  

AbstractBACKGROUND: The mechanism underlying the coexistence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) in chronic hepatitis B (CHB) patients remains unknown.
AIMS: This research aimed to determine the clinical and virological features of the rare pattern.
METHODS: A total of 32 CHB patients infected by HBV genotype C were included: 15 carrying both HBsAg and anti-HBs (group I) and 17 solely positive for HBsAg (group II). S gene and reverse transcriptase (RT) region sequences were amplified, sequenced and compared with the reference sequences.
RESULTS: The amino acid (aa) variability within major hydrophilic region (MHR), especially the "a" determinant region, and within RT for regions overlapping the MHR in group I is significantly higher than those in group II. Mutation sI126S/T within the "a" determinant was the most frequent change, and only patients from group I had the sQ129R, sG130N, sF134I, sG145R aa changes, which are known to alter immunogenicity.
CONCLUSIONS: In CHB patients, the concurrent HBsAg/anti-HBs serological profile is associated with an increased aa variability in several key areas of HBV genome. Additional research on these genetic mutants are needed to clarify their biological significance for viral persistence.
Copyright © 2015 Elsevier Editora Ltda. All rights reserved.


KEYWORDS: Chronic hepatitis B; Coexistence of HBsAg and anti-HBs; Genotype C; Reverse transcriptase; S gene; Variability

StephenW

BRAZĴ传染病杂志。 2015年21月PII：S1413-8670（15）00193-2。 DOI：10.1016 / j.bjid.2015.08.014。 [打印EPUB提前]
突变在S基因和在慢性乙型肝炎中国患者HBsAg和抗-HBs共存重叠逆转录酶区域。
丁F1，苗XL2，李YX1，戴JF1，俞HG3。
作者信息

    消化内科教研室，武汉大学人民医院，武汉，430060湖北，中国大陆的。
    教研室临床实验室，PUAI附属于华中科技大学，430060，湖北省，中国公关同济医学院附属医院。
    3Department消化内科，武汉大学人民医院，武汉，430060湖北，中国大陆的;研究所胃肠病学和肝病，武汉大学医学院，430060，湖北省，中国公关。电子地址：[email protected]。

抽象
背景：

乙型肝炎表面抗原（HBsAg）和抗体在治疗慢性乙型肝炎（CHB）的患者共存对HBsAg（抗-HBs）所依据的机制仍然是未知的。
目的：

此研究的目的是确定的稀有图案的临床和病毒学特征。
方法：

共感染HBV基因型C 32例慢性乙型肝炎患者都包括在内：1​​5携带HBsAg和抗-HBs（I组）和17只HBsAg阳性（组二）。 S基因和逆转录酶（RT）区序列进行扩增，测序，并与参考序列进行比较。
结果：

内主要亲水区（MHR）的氨基酸（AA）可变性，特别是“a”决定区域，和内RT下区域组重叠MHR我是比那些在II组显著高。突变sI126S / T中的“a”决定是最常见的变化，从组患者只有我有sQ129R，sG130N，sF134I，sG145R AA的变化，这是众所周知的改变免疫原性。
结论：

在慢性乙肝患者，并发乙肝表面抗原/抗-HBs血清学轮廓与乙肝病毒基因组的几个关键领域增加AA变异有关。因此需要对这些遗传突变体进行更多的研究，以明确其对病毒持续感染的生物学意义。

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关键词：

慢性乙型肝炎; HBsAg和抗-HBs共存; C基因型;逆转录; S基因;变化性