乙肝表面基因变异体分离自献血者显性和隐匿性HBV感染的东

StephenW

http://www.virologyj.com/content/12/1/153

Hepatitis B surface gene variants isolated from blood donors with overt and occult HBV infection in north eastern Egypt

Rania Kishk1, Nader Nemr2, Abeer Elkady34, Mohamed Mandour5, Mohamed Aboelmagd2, Nevene Ramsis5, Mohamed Hassan2, Nashaat Soliman2, Sayuki Iijima4, Shuko Murakami4, Yasuhito Tanaka4* and Mostafa Ragheb6   

    * Corresponding author: Yasuhito Tanaka [email protected]

Author Affiliations

1
Department of Microbiology and Immunology, Faculty of Medicine, Suez Canal University, El Salam District, Ismaïlia, Egypt

2
Department of Endemic and Infectious diseases, Faculty of Medicine, Suez Canal University, El Salam District, Ismaïlia, Egypt

3
Department of Clinical and Chemical Pathology, Faculty of Medicine, South Valley University, Qena, Egypt

4
Department of Virology and Liver Unit, Nagoya City University Graduate School of Medical Science, Nagoya 467-8601, Japan

5
Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, El Salam District, Ismaïlia, Egypt

6
Department of Internal Medicine, Faculty of Medicine, Aljouf University, Sakaka, KSA, Saudi Arabia

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Virology Journal 2015, 12:153  doi:10.1186/s12985-015-0389-y

The electronic version of this article is the complete one and can be found online at: http://www.virologyj.com/content/12/1/153

Received:    30 March 2015
Accepted:    21 September 2015
Published:    30 September 2015

© 2015 Kishk et al.

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Abstract
Background

Major hydrophilic region in genomic HBV extending from aa99 to aa169, clustered with a highly conformational epitope, is critical to the antigenicity of hepatitis B surface antigen (HBsAg) and may affect the diagnosis of HBV in HBV screening test. So, this study aimed to characterize variants of S gene product of hepatitis B virus (HBV) isolated from patients with overt or occult HBV infection in north-eastern Egypt.
Methods

The study included sera of two different groups of volunteer blood donors (VBDs), 82 with overt HBV that were positive for HBsAg and anti-HBc and 343 donors negative for HBsAg eligible for donation. Of the latter group, only 44 were positive for anti-HBc. All anti-HBc positive sera were subjected to HBV DNA detection and partial sequence analysis targeting the HBV S gene.
Results

HBV DNA was detected in 22.7 % of HBsAg-/anti-HBc + (10/44 patients) and in 90 % of HBsAg + donors (74/82 patients) with significant statistical difference (P = 0.0001). Phylogenetic analysis showed that HBV strains retrieved from both groups were of genotype D. Amino acid escape mutation T125M was detected in only 2 samples of the occult infection group and in none of the overt group (P = 0.01). Different amino acid substitutions were identified in overt infection group: S143L/T (16.2 %, 12/74) and P120T/S (2.7 %, 2/74). Q129R was significantly more frequent in cases with occult HBV infection (40 %, 4/10) than overt group (6.8 %, 5/74) (P = 0.01).
Conclusions

HBV genotype D predominated both in patients with overt and occult HBV infection. Different profiles of amino acid substitutions in the major hydrophilic region were seen in these two groups in Egypt.
Keywords: Occult HBV; Genotype D; Escape mutation

StephenW

乙肝表面基因变异体分离自献血者显性和隐匿性HBV感染的东北埃及

拉尼娅Kishk1，纳德Nemr2，阿比尔Elkady34，穆罕默德Mandour5，穆罕默德Aboelmagd2，Nevene Ramsis5，穆罕默德Hassan2，Nashaat Soliman2，纱幸Iijima4，修子Murakami4，保仁Tanaka4 *和穆斯塔法Ragheb6

    *通讯作者：保仁田中[email protected]

作者机构

1
微生物学与免疫学，医学系，苏伊士运河大学，El Salam酒店区，伊斯梅利亚，埃及部

2
地方病和传染病，医学系，苏伊士运河大学，El Salam酒店区，伊斯梅利亚，埃及部

3
临床部和化学病理学，医学，南谷大学，基纳，埃及学院

4
病毒学系和肝单位，医学科学的名古屋市立大学研究生院，名古屋467-8601，日本

五
临床病理科，苏伊士运河大学医学院，El Salam酒店区，伊斯梅利亚，埃及

6
内科，医学系，Aljouf大学，Sakaka，KSA，沙特阿拉伯系

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病毒学杂志2015年，12：153 DOI：10.1186 / s12985-015-0389-Y

电子版本的这篇文章是完整的一个，可以在网上找到：http://www.virologyj.com/content/12/1/153

收稿日期：2015年3月30日
接受日期：2015年9月21日
发布时间：2015年9月30日

©2015年Kishk等。

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抽象的
底

在基因组的HBV从AA99到aa169，集群具有高度构象表位延伸主要亲水区，是乙肝表面抗原的抗原性（HBsAg的）关键的，可以影响的HBV乙型肝炎病毒筛选试验的诊断。因此，本研究旨在表征乙型肝炎病毒（HBV）分离的患者有明显或隐匿性HBV感染的东北埃及的S基因产物的变体。
方法

这项研究包括两个不同的群体志愿献血者（VBD相关的），82显性乙肝病毒呈阳性HBsAg和抗-HBc和343捐助者乙肝表面抗原阴性符合捐献血清。在后一组中，只有44呈阳性抗HBc。所有抗-HBc阳性血清进行HBV DNA检测及部分序列分析针对HBV S基因。
结果

HBV DNA是在HBsAg- /抗-HBc +（10/44例）的22.7％和90％的HBsAg +捐助者（82分之74例）显著统计学差异（P = 0.0001）的检测。系统发育分析表明，这两个群体中检索HBV株的基因型D.氨基酸逃逸突变T125M只有2个样品的隐匿性感染组，并在没有任何公开组（P = 0.01）进行检测。不同氨基酸取代被确定在明显感染组：S143L / T（16.2％，74分之12）和P120T / S（2.7％，七十四分之二）。 Q129R是显著与隐匿性HBV感染（40％，4/10）较明显组（6.8％，七十四分之五）（P = 0.01）的情况下更为频繁。
结论

HBV基因D型患者的显性和隐匿性HBV感染为主两者。的氨基酸取代中的主要亲水区不同的轮廓被认为在埃及这两个群体。
关键词：隐匿性乙肝病毒; D型;逃生突变

菲凡

谢谢史蒂芬