研究人员设计出新的乙肝病毒电化学生物传感器

StephenW

Researchers design new hepatitis B virus electrochemical biosensor                   

    News ID: 2901941 - Wed 2 September 2015 - 12:03
    Technology   

TEHRAN, Sep. 2 (MNA) – A group of researchers led by Dr. M. H. Mashhadizadeh, a faculty member at Kharazmi University of Iran, developed a new hepatitis B virus (HBV-DNA) electrochemical biosensor.     Funded by Iran National Science Foundation (INSF), a new hepatitis B virus (HBV-DNA) electrochemical biosensor was designed in two phases.
In the first phase, carbon paste electrode was modified by magnetic nanoparticles, para-nitroaniline and Hepatitis B virus DNA probe. Immobilization of DNA probe onto electrode surface was done through covalent interaction between NH2 group on the electrode surface and activated phosphate group of DNA. In this work, Methylene Blue was used as electrochemical indicator and quantitative determination was done by differential pulse voltammetry (DPV) method.
In the second phase, gold electrode was modified with gold nanoparticles and mercaptobenzaldehyde. Hepatitis B virus DNA probe that had been functionalized with NH2 was immobilized onto electrode surface through covalent interaction with aldehyde group. The DNA probe immobilization and hybridization on the modified electrode were studied with differential pulse voltammetry (DPV) using ferricyanide as an indicating probe.
Evaluation of the biosensor’s performance showed appropriate sensitivity and selectivity for determining trace amounts of Hepatitis B virus DNA in the real samples. Furthermore, the biosensor was developed with robust stability and desirable reproducibility.
            

StephenW

研究人员设计出新的乙肝病毒电化学生物传感器
DNA
新闻编号：2901941 - 星期三2015年9月2日 - 12:03
技术
新华社德黑兰9月2日（MNA） - 一组研究人员领导的MH Mashhadizadeh博士，任教于伊朗Kharazmi大学，开发了一种新的乙型肝炎病毒（HBV-DNA）电化学生物传感器。

伊朗国家科学基金会（INSF），一个新的乙型肝炎病毒（HBV-DNA）资助电化学生物传感器被设计在两个阶段。

在第一阶段中，碳糊电极是由磁性纳米颗粒，对 - 硝基苯胺和乙型肝炎病毒DNA探针修饰。 DNA探针的固定化到电极表面通过在电极表面NH 2基团和DNA的活化磷酸基团之间的共价相互作用完成。在这项工作中，亚甲基蓝被用作电化学指示剂和定量测定是微分脉冲伏安法（DPV）方法完成的。

在第二阶段中，金电极金纳米粒子修饰和巯基苯甲醛。已被官能化NH 2乙型肝炎病毒DNA探针通过与醛基共价相互作用固定在电极表面上。 DNA探针固定和杂交的修饰电极，研究了用铁氰化钾作为一个指示探头差分脉冲伏安法（DPV）。

评价生物传感器的性能表现出适当的灵敏度和选择性，用于确定实际样品中的B型肝炎病毒DNA的微量。此外，生物传感器具有强大的稳定性和重现理想的发展。

MP4

一个伊朗，一个古巴，都是被美国长期封锁的国家，望世界和平

19721108

难道伊朗也要逆袭

默然10

大中华科学院士什么时候给力点啊。都说中国生物化工强，拿不出点东西不是打脸吗？