[studyforhope] 1 IU HBVDNA, 1 IU HBsAg的意义

StephenW

[studyforhope] meaning of 1 iu hbvdna 1 iu of HBsAg
Hbv DNA copy number basically is an indirect count of virions, Dane particles of 42 nm diameter in plasma.
It is determined by comparing a known standards signal after the pcr with the samples signal intensity.
Since the so called absolute standards are variable between labs, an international standard is sent to labs and used as a calibrator. This eliminates those variations, since mainly the calibrator determines the results.
But it is basically still a particle count, so that each iu represents between 3 to 6 virions.

The Quant hbsag does not directly count the HBsAg particles, which have a diameter of 22nd and represent each a sphere in which many hbsag protein monomers are embedded in a double layer lipid membrane, into which they were inserted during their synthesis at the endoplasmatic reticulum membrane, penetrating the membrane 4 times with hydrophobic alpha helixes. These monomers touch each other sideways and finally force the membrane into a spherical configuration, then pinch of as vesicles into the lumen of the ER. From here they are translocated via the golgi to the outside of the hepatocytes and the spheres are released into the blood stream.

What is measured is the combined weight or mass, not the particle number in the hbsag assays.
In the earlier days 1ng/ml was the standardized unit, also called Paul ehrlich units, developed by prof wolfram gerlichs lab.
Later this was replaced with an international standard to be sent to all labs, to accommodate all the variations in the procedures.  The sample is basically compared with the international calibrator. One iu is roughly still close to 1ng/ml.

The absolute amount of hbsag particles contained in 1 iu is still about 100 million individual particles or spheres. Most assays cutoff is about 0.05iu/ml or 5 million particles per ml. The most sensitive ones cutoff is about 1 million hbsag particles/ml.

Thus comparing DNA iUs or copies with hbsag iUs is not meaningful and the assumption that they should be one to one in terms of molecular copies is wrong by a factor of one million.
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StephenW

[studyforhope] 1 IU HBVDNA 1 IU HBsAg的意义
HBV DNA拷贝数主要是病毒粒子的间接计数，42纳米直径在血浆中Dane颗粒。
它是由PCR后进行比较的已知标准信号与所述采样的信号的强度来确定。
由于所谓的绝对标准是实验室之间可变的，一个国际标准被发送到实验室和用作校准器。这消除了这些变型中，因为主要的校准确定的结果。
但它基本上还是一个颗粒数，以使每个IU 3至6个病毒粒子之间表示。

的定量的HBsAg不直接计数HBsAg颗粒，其中有一个直径22日和表示每个球体，其中许多的HBsAg蛋白单体嵌入在双层脂质膜，在其中它们被它们的合成过程中的内质网的膜插入，穿透膜4次，疏水α螺旋


这些单体互相接触侧身，最后迫使膜成为一个球形结构，再捏作为囊泡进入内质网的内腔中。从这里它们通过高尔基体转运到肝细胞的外部，球体被释放到血液中。

什么被测量是组合重量或质量，而不是在HBsAg的测定中的粒子数。
在早期的日子为1ng / ml的是标准化的单位，也被称为艾氏保单位，由教授钨gerlichs实验室开发。
稍后被替换为一个国际标准被发送到所有的实验室，以适应在过程的所有版本。样品基本上与国际校准比较。一个IU大致仍接近为1ng / ml的。

HBsAg颗粒中含有的1国际单位的绝对量仍约为100万单个粒子或球体。大多数分析截止为每毫升约0.05iu /毫升或5万的粒子，最敏感的人截止约100万HBsAg颗粒/毫升。

因此比较的DNA IUS或用HBsAg IUS拷贝是没有意义，他们应该是一对一在分子拷贝方面的假设是错误的一百万的一个因素。

君看一叶舟

就是1:1百万之间关系，不是一个单位，相比无意义