在干扰素α对Toll样受体9信号通路体外免疫调节活性在慢性乙

StephenW

In Vitro Immunomodulatory Activity of Interferon Alpha on Toll-Like Receptor 9 Signaling Pathway in Chronic Hepatitis B

To cite this article:
Wang Xin, Zhao Ying-Ren, Liu Hong-Li, Ma Xiao-Hua, and Zhang Yu. Journal of Interferon & Cytokine Research. May 2015, 35(5): 385-391. doi:10.1089/jir.2014.0182.

Published in Volume: 35 Issue 5: May 5, 2015
Online Ahead of Print: December 23, 2014

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Author information
Xin Wang,1 Ying-Ren Zhao,1 Hong-Li Liu,2 Xiao-Hua Ma,2 and Yu Zhang1
1Department of Infectious Diseases, The First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, P.R. China.
2The Eighth Hospital of Xi'an, Xi'an, P.R. China.
Address correspondence to:
Dr. Xin Wang
Department of Infectious Diseases
The First Affiliated Hospital of Medical College of Xi'an Jiaotong University
277 Yanta West Road
Xi'an 710061
Shaanxi Province
P.R. China
E-mail: [email protected]
Received 28 September 2014
Accepted 16 October 2014
ABSTRACT

Interferon alpha (IFN-α) is registered for chronic hepatitis B (CHB) treatment. However, the antiviral mechanism of IFN-α and the biological function of many IFN-α responsive genes have not been fully elucidated. We investigated to determine the regulative effect of IFN-α on toll-like receptor (TLR) 9 signaling in peripheral blood mononuclear cells (PBMCs) from CHB patients in vitro. We examined the changes of expression and function of TLR9 signaling pathway in the PBMCs with different treatment methods and investigated the synergism of IFN-α and TLR9 ligand on antiviral cytokine secretions in vitro. The data showed that, for the TLR9 signaling pathway, IFN-α not only augmented the expressions of TLR9 signal transduction molecules but also activated the TLR9 signal function. This study has clearly demonstrated that the TLR9 ligand could stimulate PBMCs that have been pretreated with IFN-α. Furthermore, the quantity of antiviral cytokines secreted by the pretreated PBMCs was greater than those without pretreatment. The interaction between IFN-α and TLR9 ligand appears to be synergistic. Data revealed IFN-α could influence TLR9 signaling transduction and synergistically improve the immune efficacy of TLR9 ligand against CHB. The present study suggests a potential novel mechanism for the antiviral activity against hepatitis B virus and a new individualized antiviral strategy.

StephenW

在干扰素α对Toll样受体9信号通路体外免疫调节活性在慢性乙型肝炎

举这篇文章：
汪鑫，赵莹仁，刘宏丽，马晓华，和张钰。杂志干扰素与细胞因子研究。 2015年5月，35（5）：385-391。 DOI：10.1089 / jir.2014.0182。

发表在卷：35问题5：2015年5月5日
在线超前打印：2014年12月23日

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作者信息
汪鑫，1应仁兆，1宏丽刘2小华马2和俞Zhang1
传染病教研室，医学院西安交通大学，西安，中国公关第一附属医院。
西安，西安，中国P.R.的2The第八医院。
通讯地址：
王昕博士
传染病科
西安交通大学医学院第一附属医院
雁塔西路277
陕西西安710061
陕西省
P.R.中国
电子信箱：[email protected]
收到2014年9月28日
接受2014年10月16日
摘要

干扰素α（IFN-α）被注册为慢性乙型肝炎（CHB）的治疗。然而，IFN-α和许多IFN-α应答基因的生物功能的抗病毒机制尚未完全阐明。我们调查，以确定IFN-α对Toll样受体（TLR）9信号外周血单个核细胞（PBMC）从CHB患者体外的调控作用。我们研究了表达和TLR9信号传导途径的功能，在PBMC中具有不同的治疗方法的变化并调查IFN-α和TLR9配体的协同作用的体外抗病毒细胞因子的分泌物。该数据表明，对于TLR9信号传导途径，IFN-α不仅增加TLR9信号转导分子的表达，而且激活T​​LR9信号的功能。这项研究清楚地表明，TLR9配体可刺激已预先用IFN-α外周血单个核细胞。此外，通过将预处理的PBMC分泌的抗病毒细胞因子的数量大于那些没有预处理。 IFN-α和TLR9配体之间的相互作用似乎是协同的。数据显示IFN-α可能影响TLR9信号转导和协同提高对TLR9配慢性乙型肝炎的免疫效果。本研究表明一个潜在的新的机构，用于抗乙型肝炎病毒的抗病毒活性和新的个性化的抗病毒策略。