白细胞介素21的反应在慢性乙型肝炎患者

StephenW

Interleukin-21 Responses in Patients with Chronic Hepatitis B

To cite this article:
Li Jie, Ren Wanhua, Ma Wei, Zhang Jiao, Shi Jun, and Qin Chengyong. Journal of Interferon & Cytokine Research. February 2015, 35(2): 134-142. doi:10.1089/jir.2013.0119.

Published in Volume: 35 Issue 2: September 22, 2014
Online Ahead of Print: September 22, 2014


Author information
Jie Li,1,* Wanhua Ren,1,* Wei Ma,2,* Jiao Zhang,1 Jun Shi,1 and Chengyong Qin3
1Department of Infectious Disease, Shandong Provincial Hospital Affiliated to Shandong University, Shandong, China.
2Department of Oncological Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Shandong, China.
3Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, Shandong, China.

*These authors contributed equally to this work.
Address correspondence to:
Dr. Jie Li
Department of Infectious Disease
Provincial Hospital Affiliated to Shandong University
324 Jingwu Road
Ji'nan
Shandong 250021
China
E-mail: [email protected]
Received 3 November 2013
Accepted 24 July 2014
ABSTRACT

Interleukin (IL)-21 has been demonstrated to play a pivotal role in controlling chronic viral infections. However, little is known about the regulatory role of IL-21 in T cell immunity during the process of chronic hepatitis B (CHB). In the present study, the levels of serum IL-21 in 77 patients with various degrees of CHB in immune clearance phase (IC), 25 patients infected with hepatitis B virus (HBV) in immune tolerance phase (IT), and 25 healthy controls (HC) were measured and their potential association with major clinic indexes was examined. Peripheral blood mononuclear cells from CHB patients were stimulated with hepatitis B core antigen (HBcAg) in the presence or absence of anti-IL-21 antibody or recombinant IL-21, and the frequency of HBcAg-specific IL-21+CD4+ and interferon (IFN)-γ+CD8+ T cells was characterized by flow cytometry. Our data indicated that the levels of serum IL-21 were significantly higher in the IC CHB patients than that in the other groups and were positively correlated with the levels of serum HBV DNA and HBeAg in the IC patients. There was a low frequency of HBcAg-specific IL-21+CD4+ T cells in IC CHB patients. Further, IL-21 enhanced HBcAg-specific IFN-γ+CD8+ T cell proliferation, while treatment with anti-IL-21 inhibited antigen-specific IFN-γ+CD8+ T cell expansion in vitro. Our findings imply that IL-21 positively regulates proinflammatory IFN-γ+CD8+ T cell responses during the process of chronic HBV infection in humans.

StephenW

Translate from: English
白细胞介素21反应的慢性乙型肝炎患者

举这篇文章：
李杰任万华，马炜，章其嗷，石俊和秦称慵。杂志干扰素与细胞因子研究。 2015年2月，35（2）：134-142。 DOI：10.1089/ jir.2013.0119。

发表在卷：35第2期：2014年9月22日
在线超前打印：2014年9月22日


作者信息
李洁，1，万华任*，1，*卫呒呵，2，*姣查嗯，石军1，1和王成勇Qin3
传染病，山东省立医院附属教研室山东大学，山东，中国。
教研室肿瘤学外科，山东省附属山东大学，山东，中国医院。
3department消化内科，山东省附属山东大学，山东，中国医院。

*这些作者贡献同样对这项工作。
通信地址：
博士李洁
传染病科
省立山东大学附属医院
精武路324
济南
山东250021
中国
电子邮件：[email protected]
收到的2013年11月3日
接受2014年7月24日
摘要

白细胞介素（IL）-21已被证明在控制慢性病毒感染中发挥关键作用。然而，知之甚少的IL-21的T细胞免疫治疗慢性乙型肝炎（CHB）的过程中的调节作用。在本研究中，血清中IL-21的77例在免疫清除期不同程度的CHB的集成电路（IC），25例感染乙型肝炎病毒（HBV）在免疫耐受阶段（IT），和25名健康对照的水平（HC）进行测定及其与主要股指诊所潜在的关联进行了检查。从慢性乙型肝炎患者的外周血单核细胞用乙型肝炎核心抗原（HBcAg的）的存在或不存在抗IL-21抗体或重组IL-21，以及核心抗原特异性的频率的IL-21+ CD4 +和干扰素（ IFN）-γ+ CD8 + T细胞进行了表征通过流式细胞术。我们的数据表明，血清中IL-21的水平显著高于在IC CHB患者比其他组，并与血清HBV DNA和HBeAg在IC患者的水平呈正相关。有核心抗原特异性的IL-21+ CD4 + T细胞在慢性乙型肝炎患者的IC的低频率。此外，IL-21增强的HBcAg特异性IFN-γ+ CD8 + T细胞的增殖，而用抗IL-21抑制抗原特异性IFN-γ+ CD8 + T细胞扩增的体外治疗。我们的研究结果暗示了IL-21的慢性HBV感染在人中的过程中正调节促炎细胞的IFN-γ+ CD8+ T细胞应答。

王震宇

大头儿子,小头爸爸.
一对好朋友,快乐父子俩.
儿子的头大手儿小,
爸爸的头小手儿大.
大手牵小手,走路不怕滑.
走着走着走走走走,
转眼儿子就长大.