乙型肝炎病毒相关的肝癌是由微小RNA - 181a的诱导

StephenW

Hepatitis B Virus-Related Hepatocellular Carcinoma is Induced by MicroRNA-181a                                               

                        Posted by: Maureen Newman                                        February 17, 2014       

                                                                                                                        Researchers at Chongqing Medical University in China broke new ground on an association between microRNAs and hepatocellular carcinoma (HCC), the third leading cause of cancer-related deaths worldwide. The link between the two is formed by chronic hepatitis B virus (HBV), the most prominent cause (accounting for 55% of cases) for HCC. The researchers wanted to better understand the molecular mechanisms of HBV-related HCC, and they knew from previous work that miRNA-181a is up-regulated in HBV-expressing hepatocarcinoma cells, so they investigated miRNA expression in HCC cells. The results were published today in BioMed Central Cancer.
Lead author Chengcheng Zou and colleagues first transfected HBV-negative HCC cells to stably express HBV, then compared the amount of miRNA-181a production among HCC-HBV+, HCC-HBV-, and constitutively-HBV+ cells. As expected, the level of miRNA-181a expression was higher in both HBV+ cells than in HBV-cells. To investigate the implications of increased miRNA-181a, the scientists measured cell proliferation and identified that miRNA-181a-high cell number was approximately 1.3-times as large as miRNA-181a-low cell number. It was then identified that miRNA targets and inhibits E2F5, a transcription factor that negatively regulates the cell cycle, allowing the cells to leave G0 and begin proliferating. Cell proliferation and cancer go hand-in-hand, and it was next identified that miRNA-181a+ cells created larger tumors than miRNA-181a- cells when injected into mice.
These findings provide further evidence that HBV plays a role in promoting cell growth, and it may be due to miRNA-181a expression that drives cells into the cell cycle. Although more research is necessary to fully understand the relationship among miRNA-181a, HBV, and HCC, this work may drive a new therapy for stopping the progression of HCC through suppressing miRNA-181a .
This work was supported by The Major National S&T program (2013ZX10002002, ALH), Major project of Chongqing Science & Technology Commission (cstc2013jcyjC10002, ALH), and Natural Science Foundation Project of CQ CSTC (2010BB5359).

StephenW

乙型肝炎病毒相关的肝癌是由微小RNA - 181a的诱导

发布者：莫琳·纽曼2014年2月17日

MicroRNAResearchers在重庆医科大学在中国有新的突破对microRNA和肝细胞癌（HCC ） ，世界范围内癌症相关死亡的第三大原因之间的关联。两者之间的联系是由慢性乙型肝炎病毒（HBV ）形成，最突出的原因（占病例总数的55％）用于肝癌。研究人员希望更好地了解HBV相关HCC的分子机制，并从他们以前的工作知道的miRNA - 181a的上调HBV表达肝癌细胞，因此它们在肝癌细胞中研究了miRNA的表达。结果今天发表在生物医学中心癌症。

主要作者澄城邹和他的同事第一次转染HBV阴性肝癌细胞稳定表达乙肝病毒，然后比较了HCC- HBV + HCC-乙肝病毒，并组成乙肝病毒+细胞的miRNA - 181a的生产量。如预期的miRNA - 181a的表达水平明显高于在两种HBV +细胞比HBV-细胞。调查增加了miRNA的181A的影响，科学家们测量细胞的增殖，并确定了miRNA - 181a的高细胞数约为1.3倍大的miRNA - 181a的低细胞数。然后将识别出的miRNA靶基因并抑制E2F5 ，负调节细胞周期的转录因子，使细胞离开G0并开始增殖。细胞增殖和癌症的去手牵手，和它旁边发现了miRNA - 181A +细胞产生比的miRNA - 181A -细胞肿瘤较大时，注射到小鼠体内。

这些发现提供了进一步的证据表明HBV在促进细胞生长的作用，并且它可能是由于驱动细胞进入细胞周期的miRNA - 181a的表达。虽然越来越多的研究是必要的，充分了解其中的miRNA - 181A ，乙肝和肝癌的关系，这项工作可能推动通过抑制的miRNA - 181a的停止肝癌的进展的新疗法。
这项工作得到了国家重大科技计划（ 2013ZX10002002 ， ALH ） ，重庆市科学技术委员会（ cstc2013jcyjC10002 ， ALH ）的重大项目，和CQ CSTC自然科学基金项目（ 2010BB5359 ）的支持。