树鼩（树鼩）持续感染乙肝病毒在肝组织病理学变化

StephenW

Histopathological changes in the liver of tree shrew (Tupaia belangeri chinensis) persistently infected with hepatitis B virus

Ping Ruan, Chun Yang, Jianjia Su, Ji Cao, Chao Ou, Chengpiao Luo, Yanping Tang, Qi Wang, Fang Yang, Junlin Shi, Xiaoxu Lu, Linqun Zhu, Hong Qin, Wen Sun, Yuanzhi Lao and Yuan Li   

Author Affiliations

Email: Ping Ruan [email protected] - Chun Yang [email protected] - Jianjia Su [email protected] - Ji Cao [email protected] - Chao Ou [email protected] - Chengpiao Luo [email protected] - Yanping Tang [email protected] - Qi Wang [email protected] - Fang Yang [email protected] - Junlin Shi [email protected] - Xiaoxu Lu [email protected] - Linqun Zhu [email protected] - Hong Qin [email protected] - Wen Sun [email protected] - Yuanzhi Lao [email protected] - Yuan Li [email protected]

Virology Journal 2013, 10:333  doi:10.1186/1743-422X-10-333
Published: 12 November 2013
Abstract (provisional)
Background

An animal model for HBV that more closely approximates the disease in humans is needed. The tree shrew (Tupaia belangeri) is closely related to primates and susceptible to HBV. We previously established that neonatal tree shrews can be persistently infected with HBV in vivo, and here present a six year follow-up histopathological study of these animals.
Methods

Group A consists of six tree shrews with persistent HBV infection, group B consists of three tree shrews with suspected persistent HBV infection, while group C consists of four tree shrews free of HBV infection. Serum and liver tissues samples were collected periodically from all animals. HBV antigen and HBV antibodies were detected by ELISA and/or TRFIA. HBV DNA in serum and in liver biopsies was measured by FQ-PCR. Liver biopsies were applied for general histopathologic observation and scoring, immunohistochemical detections of HBsAg and HBcAg, and ultrastructural observation with electron microscope technique.
Results

Hydropic, fatty and eosinophilic degeneration of hepatocytes, lymphocytic infiltration and hyperplasia of small bile ducts in the portal area were observed in group A. One animal infected with HBV for over six years showed multiple necrotic areas which had fused to form bridging necrosis and fibrosis, and megalocytosis. The hepatic histopathological scores of group A were higher than those of group B and C. The histopathological score correlated positively with the duration of infection.
Conclusions

Hepatic histopathological changes observed in chronically HBV-infected tree shrews are similar to those observed in HBV-infected humans. The tree shrew may represent a novel animal model for HBV infection.

StephenW

树鼩（树鼩）持续感染乙肝病毒在肝组织病理学变化

，姬曹蒹葭苏平阮经天，杨淳，晁区，罗Chengpiao ，延平唐，王奇，方杨，君临施，小徐路，朱Linqun ，康勤，闻孙，远致老挝和袁立

作者单位

电子邮件：阮平[email protected] - 骏扬[email protected] - 葭苏[email protected] - 吉曹[email protected] - 超欧[email protected] - 罗Chengpiao luochengpiao @ 163.com - 延平唐[email protected] - 王奇[email protected] - 方阳[email protected] - 君临世[email protected] - 晓旭鲁[email protected] - 朱Linqun 185832372 @ QQ 。 COM - 洪沁[email protected] - 闻孙[email protected] - 远志老挝laurence.ylao @ gmail.com - 袁立[email protected]的

病毒学杂志2013 ， 10:333 DOI ： 10.1186/1743-422X-10-333
发布时间： 2013年11月12日
摘要（临时）
背景

HBV ，更接近人类疾病的动物模型是必要的。树鼩（树鼩）是密切相关的灵长类动物和易受HBV 。我们以前建立的新生树鼩可以与HBV持续感染在体内，并在这里提出了6年的随访中，这些动物的病理组织学研究。
方法

A组， B组由六个HBV持续感染树鼩由三个怀疑HBV持续感染树鼩，而C组由四个无HBV感染树鼩。定期收集所有动物血清和肝组织样本。通过ELISA和/或TRFIA检测HBV抗原和HBV抗体。 FQ - PCR测定血清HBV DNA和肝活检。肝活检应用于一般的病理组织学观察和评分，免疫组化检测HBsAg和HBcAg ，用电子显微镜技术和超微结构观察。
结果

在汇管区小胆管肝细胞的变性水肿，脂肪和嗜酸性粒细胞，淋巴细胞浸润和增生，观察A组动物HBV感染者超过六年已连成一体，形成桥接坏死和纤维化坏死区呈多发，和megalocytosis 。肝组织病理学评分A组均高于B，C组的组织病理学得分感染的持续时间呈正相关。
结论

在慢性HBV感染树鼩肝组织病理学变化观察HBV感染人类的相似。 HBV感染树鼩可能代表一种新的动物模型。