B型肝炎病毒表面抗原变异的患病率13年中国通用疫苗接种计

StephenW

Change in Hepatitis B Virus Large Surface Antigen Variant Prevalence 13 Years after Implementation of a Universal Vaccination Program in China

    Tao Bian a,b,
    Hongxia Yan a,c,
    Liping Shen a,
    Feng Wang a,
    Shuang Zhang a,
    Yanqiang Cao d,
    Shuo Zhang a,
    Yong Zhang a and
    Shengli Bi a

- Author Affiliations

    National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping District, Beijing, Chinaa
    Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina, USAb
    Bureau for Health Inspection and Supervision of Chaoyang District, Chaoyang District, Beijing, Chinac
    Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang, Chinad

ABSTRACT

A nationwide hepatitis B virus (HBV) vaccination program was implemented in China starting in 1992. To study the change in HBV variant prevalence with massive immunization, large HBV surface protein (LHBs) genes from HBV surface antigen (HBsAg)-positive sera were amplified and sequenced. The prevalences of LHBs mutants were compared between the 1992 and 2005 surveys in child and adult groups. The prevalence of “α” determinant mutants in the children increased from 6.5% in 1992 to 14.8% in 2005, where the G145R mutant occurred most frequently. In contrast, mutation frequencies showed little difference between 1992 (9.4%) and 2005 (9.9%) in adults. Moreover, compared to the 1992 survey, the child group surface (S) protein mutation frequency specifically increased (P = 0.005) in the 2005 survey, but the pre-S region mutation frequency did not show a significant difference (P > 0.05). However, the mutation frequency in the adult group increased in both the pre-S and S regions. Furthermore, the frequencies of the disease-related pre-S2 deletion and start codon mutations were significantly higher in the adult groups than in the child groups in both the 1992 and 2005 surveys (P < 0.01). Massive immunization enhances the HBV S protein mutation; the prevalence of LHBs mutants, particularly disease-related mutants, tends to increase with patient age.

StephenW

B型肝炎病毒表面抗原变异的患病率13年中国通用疫苗接种计划实施后

    陶卞某， B，
    红霞艳A，C，
    黎平沉某，
    汪峰一，
    双掌一，
    曹言强D，
    张硕一，
    张勇a和
    胜利毕一

- 作者所属机构

    研究所病毒病预防控制，中国疾病预防控制中心，北京市昌平区，吃奶
    分子药剂部，埃什尔尔曼药学院，北卡罗莱纳大学教堂山分校，北卡罗莱纳州， USAB
    局卫生监督朝阳区，朝阳区，北京， Chinac的
    河北出入境检验检疫局，石家庄， Chinad

摘要

从1992年开始在中国实施一项全国性的乙肝病毒（HBV）疫苗接种计划。为了研究乙肝病毒变异的患病率随着大规模的免疫，乙肝病毒表面大蛋白（ LHBs的）的乙肝病毒表面抗原（HBsAg）阳性血清进行扩增和测序的基因变化。患病率的LHBs的突变，比较1992年和2005年的调查，儿童组和成人组。 “α”决定簇突变在儿童的患病率从1992年的6.5％上升到2005年的14.8 ％ ，其中G145R突变最常发生。相比之下，突变频率1992 （9.4％）和2005年（9.9％）在成人之间的差异很小。此外，相比1992年的调查，特别是子组表面蛋白（S）突变频率增加（P = 0.005 ） ，在2005年的调查，但前S区基因突变频率并没有表现出显着性差异（ P > 0.05 ） 。然而，在成人组的突变频率增加前S和S区。此外，疾病相关的前S2缺失和起始密码子突变频率均明显高于成人组比孩子在1992年和2005年的调查组（P <0.01 ） 。大规模的免疫增强HBV的S蛋白突变LHBs的突变体，特别是与疾病相关的突变体，患病率与患者年龄趋于增大。