病毒感染多重聚合酶链反应检测肝功能不全的患者

StephenW

http://www.docguide.com/analysis-viral-infection-multiplex-polymerase-chain-reaction-assays-patients-liver-dysfunction
Source: Intern Med  |  Posted 4 days ago
Analysis of viral infection by multiplex polymerase chain reaction assays in patients with liver dysfunction; Ito K, Shimizu N, Watanabe K, Saito T, Yoshioka Y, Sakane E, Tsunemine H, Akasaka H, Kodaka T, Takahashi T; Internal Medicine (Tokyo, Japan) 52 (2), 201-11 (2013)
病毒感染多重聚合酶链反应检测肝功能不全的患者
   
Objective: While unexplained liver dysfunction is common, it is sometimes difficult to identify its exact cause. One cause is viral infections. The identification of viruses other than hepatitis B and C that cause liver dysfunction is difficult because no methods to simultaneously identify these viruses have been established. The aim of this study was to quickly and simultaneously identify multiple virus species. Methods A total of 49 patients with unexplained liver dysfunction and undetermined inflammation were examined. The majority of patients had hematologic malignancies, and some had undergone bone marrow transplantation. Qualitative polymerase chain reactions (PCR) were performed to detect 12 species of DNA virus in whole blood. Quantitative real-time PCR was performed when a specific virus was amplified. In addition, 6 RNA hepatitis viruses were directly assayed by real-time PCR. These 2 PCR steps were completed within 1 hour. Results The most frequently detected virus in 37 patients with liver dysfunction, was transfusion transmitted virus (38%), which was followed by human herpes virus (HHV) type 6 (35%), Epstein-Barr virus (14%), cytomegalovirus (8%), and rarely hepatitis G virus and HHV-7 (3%). Similar viremia was observed in 12 patients with mild liver dysfunction. The results of the PCR assay were mostly consistent with those of routine virus serological tests. Conclusion A multiplex viral PCR assay was a useful tool for quickly identifying viruses that possibly cause liver dysfunction. It was also important that liver dysfunction acted as a proband that led to the discovery of serious viremia

StephenW

目的：不明原因的肝功能障碍是常见的，有时是很难确定其确切病因。原因之一是病毒感染。识别以外乙型和丙型肝炎病毒引起肝功能障碍是困难的，因为没有方法来同时识别这些病毒已经建立。本研究的目的是快速，同时识别多个病毒种类。方法对49例不明原因的肝功能障碍和不确定的炎症进行了检查。大多数患者血液系统恶性肿瘤，以及一些曾接受骨髓移植。定性的聚合酶链反应（PCR）进行检测全血中的12种的DNA病毒。定量实时PCR进行扩增，当一个特定的病毒。此外，有6 RNA肝炎病毒直接通过实时PCR检测。这2个PCR步骤，在1小时内完成。结果在37例肝功能不全的最常见检测到的病毒，输血传播病毒（38％），其次是人类疱疹病毒（HHV）第6类（35％），巨细胞病毒，EB病毒（14％）（ 8％），而且很少庚型肝炎病毒和HHV-7（3％）。类似的病毒血症12例轻度肝功能不全。 PCR检测的结果与那些常规病毒血清学测试大部分是一致的。结论多重病毒PCR检测是一个非常有用的工具，用于快速识别病毒可能引起肝功能障碍。同样重要的是，作为一个先证者，导致严重的病毒血症的发现肝功能障碍。