Fuzheng Huayu recipe prevents nutritional fibrosing steatohepatitis in mice

StephenW

  ResearchFuzheng Huayu recipe prevents nutritional fibrosing steatohepatitis in mice            

Yan-hong  Jia, Rong-Qi  Wang, Hong-Mei  Mi, Ling-Bo  Kong, Wei-Guang  Ren, Wen-Cong  Li, Su-Xian  Zhao, Yu-Guo  Zhang, Wen-Juan  Wu, Yue-Min  Nan and Jun  Yu        

                                                                                    
Lipids in Health and Disease 2012, 11:45 doi:10.1186/1476-511X-11-45
Published: 28 March 2012               
Abstract (provisional)Background

Fuzheng Huayu recipe (FZHY), a compound of Chinese herbal medicine, was reported to   improve liver function and fibrosis in patients with hepatitis B virus infection.   However, its effect on nutritional fibrosing steatohepatitis is unclear. We aimed   to elucidate the role and molecular mechanism of FZHY on this disorder in mice.

Methods

C57BL/6 J mice were fed with methionine-choline deficient (MCD) diet for 8 weeks to   induce fibrosing steatohepatitis. FZHY and/or heme oxygenase-1 (HO-1) chemical inducer   (hemin) were administered to mice, respectively. The effect of FZHY was assessed by   comparing the severity of hepatic injury, levels of hepatic lipid peroxides, activation   of hepatic stellate cells (HSCs) and the expression of oxidative stress, inflammatory   and fibrogenic related genes.

Results

Mice fed with MCD diet for 8 weeks showed severe hepatic injury including hepatic   steatosis, necro-inflammation and fibrosis. Administration of FZHY or hemin significantly   lowered serum levels of alanine aminotransferase, aspartate aminotransferase, reduced   hepatic oxidative stress and ameliorated hepatic inflammation and fibrosis. An additive   effect was observed in mice fed MCD supplemented with FZHY or/and hemin. These effects   were associated with down-regulation of pro-oxidative stress gene cytochrome P450   2E1, up-regulation of anti-oxidative gene HO-1; suppression of pro-inflammation genes   tumor necrosis factor alpha and interleukin-6; and inhibition of pro-fibrotic genes   including alpha-smooth muscle actin, transforming growth factor beta 1, collagen type   I (Col-1) and Col-3.

Conclusions

Our study demonstrated the protective role of FZHY in ameliorating nutritional fibrosing   steatohepatitis. The effect was mediated through regulating key genes related to oxidative   stress, inflammation and fibrogenesis.

The complete article is available as a provisional PDF. The fully formatted PDF and HTML versions are in production.         

StephenW

扶正化瘀方阻止在小鼠体内的营养性脂肪肝纤维化

贾延红，荣王齐，美公里，孔令博，任伟广，李文琮，赵苏贤，张钰郭，吴文娟，南跃民俞军


血脂在健康与疾病2012年，11:45 DOI：10.1186/1476-511X-11-45
出版日期：2012年3月28日
摘要
背景

据报道，中草药复方扶正化瘀方（FZHY）改善肝功能和乙肝病毒感染患者的肝纤维化。然而，它的营养纤维化肝炎的影响目前还不清楚。我们的目的是要澄清的作用和小鼠对这种疾病的分子机制FZHY。
方法

C57BL / 6 J小鼠饲喂蛋氨酸胆碱缺乏（MCD）的8个星期的饮食诱导纤维化肝炎。 FZHY和/或血红素加氧酶1（HO-1）的化学诱导（血红素）小鼠，分别。进行了评估的FZHY的影响比较严重肝损伤，肝脂质过氧化水平，活化肝星状细胞（造血干细胞）和氧化应激，炎症和纤维化相关基因的表达。
结果

MCD饮食喂养8周的小鼠表现出严重的肝损伤，肝脂肪变性，坏死炎症和纤维化。政府FZHY或血红素显着降低血清谷丙转氨酶，谷草转氨酶，降低肝脏的氧化应激和改进的肝脏炎症和纤维化。在小鼠喂养MCD的补充与FZHY和/或血红素添加剂的效果观察。这些影响与亲氧化应激基因的细胞色素P450 2E1，抗氧化，HO-1基因的上调调节;亲炎症基因抑制肿瘤坏死因子α和IL-6和抑制亲纤维化的基因，包括α-平滑肌肌动蛋白，转化生长因子β1，I型胶原（COL-1）和Col-3。
结论

我们的研究表明，在改善营养纤维化肝炎的FZHY的保护作用。通过调节氧化应激，炎症和纤维化有关的关键基因介导的效果。
完整的文章，是作为一个临时的PDF。在生产中完全格式化的PDF和HTML版本。

咬牙硬挺

感谢分享

非东亚病夫

中药是治不好乙肝的~~~！不要太大梦想~~！中药是食疗，而乙肝是感染免疫。所以中药是治不好乙肝的。别老是听中医痴人说梦话了。