Heat impact upon the infectivity of hepatitis B virus in serum.

StephenW

Zhonghua Yu Fang Yi Xue Za Zhi. 2011 Aug;45(8):723-6.
Heat impact upon the infectivity of hepatitis B virus in serum.
[Article in Chinese]
Source: http://www.ncbi.nlm.nih.gov/pubmed/22169694
Song XX, Ju LW, Wei GR, Jiang QW.
Source
Department of Epidemiology, College of Public Health of Fudan
University, Key Laboratory of Ministry Education of Public Health Security,
Shanghai 200032, China.

Abstract
OBJECTIVE: This article was to explore the impact of temperature on
hepatitis B virus infectivity.

METHODS: HBV positive serum with a HBV DNA titer of 1.33 × 10(8) copies/ml
was aliquoted into 23 Ep tubes with 1.5 ml, 100 µl in one tube.15 tubes
were incubated at 37, 56 and 65°C for 0, 30, 60, 120 and 600 minutes,
respectively. The other 8 tubes were incubated at 98°C for 0, 5, 10 and 30
minutes, respectively. Post-treated serum at all time points were selected
to infect HepG-2 cell. When 18 hours after infection, these cells were
extensively washed with phosphate buffered saline. Cells were harvested
after the addition of fresh culture medium to culture cells for 48 hours.
HBV DNA was detected by FQ-PCR.

RESULTS: HBV DNA was detected in cells that were infected by serum at 37°C
and 56°C for 30, 60, 120 and 600 minutes, respectively. The titers for the
cells incubated at 37°C were (4.85 ± 1.71) × 10(5), (3.85 ± 1.76) ×
10(5), (1.67 ± 0.67) × 10(5), (7.86 ± 1.03) × 10(4) copies/ml, and
those for the cells incubated at 56°C were (4.01 ± 0.16) × 10(5), (9.77
± 0.97) × 10(4), (6.36 ± 0.65) × 10(4), (5.05 ± 0.24) × 10(3)
copies/ml at different incubation time points. For the cells incubated at
65°C for 60 and 120 minutes, HBV DNAs were (5.15 ± 7.28) × 10(3) and
(7.56 ± 10.60) × 10(2) copies/ml, respectively, which were much lower
than those in the controls cells ((6.79 ± 1.48) × 10(5) copies/ml). The
results of HBV DNA were different (F = 104.4, P < 0.001) in groups treated
with different temperature, and results of HBV DNA were also different (F =
144.0, P < 0.001) in groups processed for different period of time.
Temperature and processing time had interaction (F = 23.6, P < 0.001).
After heating at 98°C for 10 minutes and boiling for 5 minutes, the HBV
DNA copy number ((3.02 ± 4.26) × 10(2), (4.31 ± 6.09) × 10(2)
copies/ml) in infected cells decreased by about 10 folds than that in the
control group ((6.79 ± 1.48) × 10(5) copies/ml). HBV DNAs were not
detected in cells that were infected by serum which was heated at 98°C for
30 minutes and boiled for 10 minutes.

CONCLUSION: The infectivity of HBV serum in vitro was relatively stable at
low temperture, and it would lose its infectivity in short period of time
at high temperature.

StephenW

中华游方医学杂志。 2011八月45（8）:723 - 6。
热冲击后，血清中的乙肝病毒的传染性。
[在中国]
资料来源：http://www.ncbi.nlm.nih.gov/pubmed/22169694
宋XX，鞠LW，遗传资源，魏江量子阱。
SourceDepartment流行病学，复旦大学公共健康学院
大学重点实验室，教育部公共卫生安全教育，
上海200032，中国。

摘要
目的：本文是探讨温度的影响
B型肝炎病毒的传染性。

方法：HBV阳性血清与HBV DNA滴度为1.33 × 10（8）拷贝/ ml
被分装成23个EP管1.5毫升，100μL一tube.15管
在孵育37，56和65 ° C为0，30，60，120和600分钟，
。其他8管孵育在98 ° C为0，5，10和30
分钟，分别。治疗后各时间点血清选择
感染HEPG - 2细胞。当18个小时后感染，这些细胞
广泛用磷酸盐洗涤缓冲液。收获细胞
除了新鲜培养基培养细胞48小时后。
FQ - PCR检测HBV - DNA检测。

结果：HBV - DNA检测血清感染的细胞在37 ° C
56 ° C为30，60，120和600分钟。滴度
细胞培养在37 ° C组（4.85 ± 1.71）× 10（5），（3.85 ± 1.76）×
10（5），（1.67 ± 0.67）× 10（5），（7.86 ± 1.03）× 10（4）拷贝/ ml，并
这些细胞孵育56 ° C组（4.01 ± 0.16）× 10（5），（9.77
± 0.97）× 10（4），（6.36 ± 0.65）× 10（4），（5.05 ± 0.24）× 10（3）
在不同的孵化时间点拷贝/ ml。对于在培养的细胞
65 ° C为60和120分钟，HBV DNA的分别为（5.15 ± 7.28）× 10（3）
（7.56 ± 10.60）× 10（2）拷贝/毫升，分别是低得多
比在控制细胞（（6.79 ± 1.48）× 10（5）拷贝/ ml）。 “
HBV DNA的结果是不同的治疗（F = 104.4，P <0.001），组
不同温度下，HBV DNA的结果也不同（F =
144.0，P <0.001），组不同时间处理。
温度和处理时间交互作用（F = 23.6，P <0.001）。
后98℃加热10分钟，煮沸5分钟，乙肝病毒
DNA拷贝数（（3.02 ± 4.26）​​× 10（2），（4.31 ± 6.09）× 10（2）
在感染的细胞中的拷贝/ ml）下降了约10倍比
对照组（（6.79 ± 1.48）× 10（5）拷贝/ ml）。乙肝病毒DNA的人不
检测血清感染的细胞，这是在98 ° C下加热
30分钟，煮沸10分钟。

结论：乙肝病毒血清在体外的传染性相对稳定，
低温，在较短的时间内将失去其传染性
在高温下。