HBV genotypes B & C gentotyping and quantification

StephenW

<http://www.journalofclinicalvirology.com/article/PIIS1386653211003404/abstract?rss=yes>

Journal of Clinical Virology

Article in Press
Development of a novel genotype-specific loop-mediated isothermal
amplification technique for Hepatitis B virus genotypes B and C genotyping
and quantification

Zhejun Cai, Guoqiang Lou, Ting Cai, Jin Yang, Nanping Wu

Received 1 June 2011; received in revised form 2 August 2011; accepted 15
August 2011. published online 12 September 2011. Corrected Proof

Abstract
Background

There is the need for a rapid, economical method for genotyping Hepatitis B
virus (HBV) to support clinical practice. Objectives

To develop a novel HBV genotyping process using genotype specific loop
mediated isothermal amplification (LAMP). Study design

HBV genotypes B and C specific LAMP methods were evaluated using standard
panel. A comparative analysis of the LAMP test against Taqman assay using
105 clinical samples, was undertaken to evaluate the quantitation capacity
of the method. 111 clinical samples were used to test the clinical
applicability of the genotype specific LAMP method. The results were
compared with those obtained by real-time PCR based genotyping and
sequencing. Results

Using genotype-specific primers, the LAMP assay correctly identified all
predefined genotypes B and C, and no cross-reaction was observed. Real-time
format of this assay provides simultaneous identification and
quantification of genotypes B and C. The detection sensitivity of the
method was found to be 323 and 515copies/ml for genotypes B and C specific
LAMP assay respectively. High correlation (R2=0.91) and good agreement
between the LAMP method and the real-time PCR test were achieved for HBV
quantitation. Samples from 111 HBV-infected patients were genotyped with
LAMP, revealing 53% HBV as genotype B, 36% as genotype C, and 12% as mixed
genotypes B and C. LAMP method showed coincidence rates of 96.7% with the
real-time PCR genotyping results.

Conclusion
This approach is a promising tool for HBV genotyping and quantitation. It
appears to be useful for routine clinical practice even in field
investigation.

StephenW

谷歌翻译
不是100％正确，仅供参考使用。

临床病毒学杂志

文章在新闻
发展一种新的基因型特定的环介导等温
B型肝炎病毒基因型B和C基因型的扩增技术
和定量

贺国强娄Zhejun蔡，蔡婷，杨进，吴南屏

2011年6月1日，修订2011年8月2日收到;接受15
2011年8月。在网上公布2011年9月12日。更正的证明

摘要
背景

是需要一种快速，经济的基因分型B型肝炎的方法
病毒（HBV），以支持临床实践。目标

为了开发一种新的乙肝病毒基因分型的过程中使用的基因型特定的循环
介导等温扩增（LAMP）。研究设计

乙肝病毒基因型B和C的具体灯泡方法是使用标准的评价
面板。对TaqMan探针检测使用一个灯测试的比较分析
105，临床样品进行了评估的定量分析能力
的方法。 111个临床样本被用来测试的临床
基因型特定的灯法的适用性。结果
比获得实时PCR为基础的基因分型
测序。结果

使用基因型特异性引物，LAMP检测正确识别所有
预定义的基因型B和C，无交叉反应进行了观察。实时
这个实验的格式，同时提供识别和
量化的基因型B和C的检测灵敏度
方法被发现是323和基因型B和C的具体515copies/ml
灯检测。很高的相关性（R2 = 0.91）和好协议
之间的LAMP方法和实时PCR检测，均达到HBV
定量。 111例HBV感染患者的样本进行基因分型与
灯53％，揭示HBV B基因型，C基因型，36％和12％为混合
基因型B和C.灯法表明，随着符合率96.7％
实时PCR基因分型结果。

结论
这种方法是一个有前途的工具HBV基因分型及定量。它
似乎为日常临床实践中有用的​​，即使在外地
调查。