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I had given some wrong interpretations on this forum about how REP 9AC works to inhibit the entry/exit of HBV. I want to share the explanations, kindly provided to me by Studyforhope on MedHelp, on how REP 9AC actually works. I don't know who Studyforhope is, but to me, he/she is very knowledgeable and I am very grateful to him/her for the explanation.
Your questions:
1.Does REP 9AC block the release/PRODUCTION/FORMATION of spherical and elongated particles(no viral dna and is non-infectious) of HBsAg from infected liver cells?
YES
2.Does REP 9AC block the release of Dane particles from infected liver cells?
NO
3. Or does REP 9AC block the formation of Dane particles?
NO
The measurement of viral load is done by extrating DNA from the lysed Dane particles. Since the HBv genome is present once per viron, a Dane particle count can be easily calculated from the DNA amount found in the patient serum.
Surface antigen is produced from the HBV cccDNA (circular, closed coiled) by transcription in the nucleus where the cccDNA resides a a minichromosome. The messenger RNA moves to the cytosol for translation and the surface antigen protein is primarily inserted (HERE IS THE BLOCK BY REPLICOR) in the endoplasmatic reticulum membrane and later processed into vesicles for export.
Dane particle HBV DNA is synthesized from the progenomic RNA ( made by transcription from the intranuclear permanent cccDNA) inside partially formed cores inside the cystosol, which close by self assembly to a closed sphere while this happens, causing the partial singlestrandedness (incompleteness) of the second so called HBV genomic plus strand. The cores are later covered with a surface antigen/membranecoat, that is fastened to the core by inward directed preS1 loops, while the outward, myristylated preS1 loops of the Dane particles are the ones that make later (after release of the virion from the hepatozyte into the circulation) contact for infection to the targeted liver cell membrane receptor, causing entry to the hepatozyte. When an artificillay introduced pres1 peptide molecule is already stuck on these receptors (importantly, a small receptor percentage is enough to block!!!) (HERE IS THE BLOCK BY MYRCLUDEX!) then the Dane particle membrane cannot get close enough to the cell membrane to FUSE, thus NO ENTRY, no reinfection, a cleaner liver, day by day....
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发表于 2011-8-13 17:15
