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<http://jmm.sgmjournals.org/content/60/7/920.abstract>
A prospective time-course study on serological testing for human
immunodeficiency virus, hepatitisB virus and hepatitis C virus with blood
samples taken up to 48 h after death
Carolin Edler1,†,
Birgit Wulff1,†,
Ann-Sophie Schröder1,
Ina Wilkemeyer2,
Susanne Polywka3,
Thomas Meyer3,
Ulrich Kalus2,† and
Axel Pruss2,†
+ Author Affiliations
1Institute of Forensic Medicine, University Hospital Hamburg-Eppendorf
(UKE), Martinistrasse, D-20246 Hamburg, Germany
2University Tissue Bank, Institute of TransfusionMedicine, Charité –
Universitätsmedizin Berlin, Charitéplatz 1,D-10117 Berlin, Germany
3Institute of Medical Microbiology, Virology andHygiene, University
Hospital Hamburg-Eppendorf (UKE), Martinistrasse,D-20246 Hamburg, Germany
Correspondence
Axel Pruss [email protected]
Received 4 November 2010.
Accepted 22 March 2011.
Abstract
The transmission of viral and non-viral infectious pathogens continues to
be the most serious of the potential adverse effects of allogenic tissue
transplantations. EU Directive 2006/17/EC stipulates that cadaveric blood
specimens for serology testing in the context of post-mortem tissue
donation must be taken not later than 24 h post-mortem. An expanded time
slot would significantly improve the availability of tissue donations, but
there are no significant data on the stability of infectious serology
assays for anti-human immunodeficiency virus (HIV), anti-hepatitis C virus
(HCV), hepatitis B virus (HBV) surface antigen (HBsAg) and anti-HBC core
antigen (HBc) in samples collected more than 24 h post-mortem. In this
prospective study, serum samples of 30 deceased persons were taken upon
admission to the Institute of Forensic Medicine (University Hospital
Hamburg-Eppendorf, Germany) and at 12,24, 36 and 48 h post-mortem. All
samples were measured twice, first using the Abbott AxSYM system, and then
after ~9 months of storage at −70 °C using the BEP III System with
Siemens and Ortho reagents. For HIV, six deceasedpersons with a pre-mortem
HIV history were included. All samples (at committal and at 12, 24, 36, 48
h) were reactive. Indeterminate or false-negative results did not occur.
For HCV, 17 deceased persons with a pre-mortem HCV history were included;
16 samples were reactive up to 48 h and one was reactive at 36 h
post-mortem (48 h sample was not available). Indeterminate or
false-negative results did not occur. For HBV, nine deceased persons were
included: five samples were initially positive for HBsAg and remained
positive up to 48 h, and eight of the samples were reactive for anti-HBc up
to 48 h and one up to 36 h post-mortem (48 h sample was not available).
Indeterminate or false-negative results did not occur. These data suggest
that infectious serological testing may be extended for blood samples of
potential tissue donors collected up to 48 h post-mortem to detect
antibodies or antigens for HIV, HBV and HCV.
↵† These authors share first and seniorauthorship. 一个准时间进程研究人类血清学检测
免疫缺陷病毒，乙型肝炎病毒和丙型肝炎病毒的血液
采取了48小时后死亡样本
Carolin Edler1，†，
吉特Wulff1，†，
安 - 索菲Schröder1，
伊娜Wilkemeyer2，
苏珊Polywka3，
托马斯Meyer3，
乌尔里希Kalus2，†和
阿克塞尔Pruss2，†
+作者作者单位
1Institute法医，大学医院汉堡Eppendorf公司
（UKE），Martinistrasse，D - 20246汉堡，德国
2University组织银行，输血医学，Charité研究所 -
Universitätsmedizin柏林，Charitéplatz 1，D - 10117柏林，德国
3Institute医学微生物学，病毒学和生，大学
医院汉堡Eppendorf公司（UKE），Martinistrasse，D - 20246汉堡，德国
信件
阿克塞尔Pruss axel.pruss @ charite.de
收到2010年11月4日。
接受2011年3月22日。
摘要
病毒性和非感染性病原体的传播病毒的继续
是的异体组织的潜在不利影响最严重的
移植。欧盟指令2006/17/EC规定，尸体的血液
对血清学检测标本中的尸体解剖组织中
捐赠必须注意不要迟于24小时后验。扩大的时间
槽将大大提高组织捐赠的可用性，但
有对传染性血清学稳定性没有显着的数据
为抗人免疫缺陷病毒检测（HIV），抗丙型肝炎病毒
（HCV），乙型肝炎病毒（HBV）表面抗原（HBsAg）和抗- HBC核心
样品中抗原（HBC）收集超过24小时后验。在这
未来的研究中，30死者血清样本被送往经
入场，法医（大学医院研究所
汉堡Eppendorf公司，德国），12，24，36和48 h后验。所有
样品测定两次，第一次使用雅培AXSYM系统，然后
后〜9个月的储存在-70 ° C使用BEP III系统与
西门子和Ortho试剂。对于艾滋病，六个预验死者
艾滋病毒的历史都包括在内。所有样品（在拘押和12，24，36，48
h）分别为反应。不定或假阴性结果并没有出现。
对于HCV，17预验HCV历史死者被包括在内;
16样品反应达48小时，1人在36小时反应
验尸报告（48小时样品不可用）。不确定或
假阴性结果并没有出现。对于乙肝，九人死亡
包括：五个样本最初为乙肝表面抗原阳性和保持
积极可达48小时，而样品为八人无抗- HBc起来
48个小时，一上来就36小时后验（48小时样品不可用）。
不定或假阴性结果并没有出现。这些数据表明
该传染病血清学检测可以延长血样
潜在的组织收集了48个小时后，验尸发现捐赠者
抗体或艾滋病毒，乙型肝炎病毒和丙型肝炎病毒抗原。
↵†这些作者占有率第一和高级作者。
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发表于 2011-6-20 14:36
