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乙肝表面抗原检测方法最初在20世纪70年代使用radioimmunoassays描述和酶免疫测定[21,22]。从那时起，各种诊断技术已被开发出来，其中大多局限于定性诊断乙肝在临床实践中。最近，乙肝表面抗原定量检测已经开发，检测和两个商用这里将简要介绍。
Methods to detect HBsAg were first described in the 1970susing radioimmunoassays and enzyme immunoassays[21,22]. Since then, various diagnostic techniqueshave been developed, which are mostly confined to qualitatively diagnose HBV inclinical practice. Recently, quantitative assay of HBsAg has been developed,and two commercially available assays will be briefly introduced here.
Architect乙肝表面抗原的QT（雅培诊断，威斯巴登，德国），是一个化学发光微粒子免疫分析法，这是目前最广泛的临床研究使用的方法[23]。
ArchitectQT检测乙肝表面抗原是一个灵活的检测协议两步免疫，简称Chemiflex，为定量测定人血清乙肝表面抗原和血浆浓度。在第一个步骤，样品和乙肝表面抗原抗体（抗- HBs）与顺磁性微粒涂层相结合。样品中乙肝表面抗原结合到抗- HBs涂层微粒。洗涤后，acridinium标记的抗- HBs的共轭被添加。接着一个洗涤周期，预触发和触发解决方案加入到反应混合物。由此产生的化学发光反应的相对计量（RLUs）光单位。在样品表面抗原量和RLUs由Architect免疫系统光学检测间存在直接的关系。ArchitectHBsAg的是一个完全自动化的系统，可探测到0.2毫微克/低的0.05-250.0与国际单位/毫升[24]动态范围乙肝表面抗原毫升。
The Architect HBsAg QT (Abbott Diagnostic, Wiesbaden, Germany)is a chemiluminescent microparticle immunoassay, which is currently the methodmost widely used in clinical studies[23]. The Architect HBsAg QT assay is a two-stepimmunoassay with flexible assay protocols, referred to as Chemiflex, forquantitatively determining human serum and plasma HBsAg concentrations. In thefirst step, the sample and hepatitis B surface antigen antibody (anti-HBs)coated with paramagnetic microparticles are combined. HBsAg present in thesample binds to the anti-HBs coated microparticles. After washing,acridinium-labeled anti-HBs conjugate is added. Following another wash cycle,pre-trigger and trigger solutions are added to the reaction mixture. Theresulting chemiluminescent reaction is measured as relative light units (RLUs).A direct relationship exists between the amount of HBsAg in the sample and theRLUs detected by the Architect Immunoassay System optics. The Architect HBsAgis a fully automated system and can detect as low as 0.2 ng/mL of HBsAg with adynamic range of 0.05-250.0 IU/mL[24].
Elecsys第二乙肝表面抗原（罗氏诊断，印第安纳波利斯，在美国）是另一种定量测定乙肝表面抗原[25]的方法。在第一个孵育阶段，在样本抗原反应，形成一个复杂的两个生物素三明治乙肝表面抗原单克隆特异性抗体和单克隆/多克隆（羊）乙肝表面抗原特异性抗体与钌配合物标记，。在第二个步骤，链涂层微粒添加，并通过与霉亲和固相相互作用的复杂结合。
结果报告为截止指数（信号采样/截止），样品被认为是反应，如果该指数大于1.0。 Elecsys HBsAg II (Roche Diagnostics, Indianapolis, IN, USA) is another method forquantitatively determining HBsAg[25]. In the first incubation step, the antigenin the sample reacts with two biotinylated monoclonal HBsAg-specific antibodiesand a monoclonal/polyclonal (sheep) HBsAg-specific antibody, labeled with aruthenium complex, to form a sandwich complex. In the second step,streptavidin-coated microparticles are added, and the complex binds to thesolid phase via interaction with biotin and streptavidin. The resultsare reported as a cutoff index (signal sample/cutoff), and the sample isconsidered reactive if the index is greater than 1.0.
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发表于 2011-6-8 18:32
