本帖最后由 StephenW 于 2011-6-7 13:30 编辑
http://www.gastrojournal.org/article/S0016-5085%2811%2900269-1/abstract
Hepatitis B Virus Limits Response of Human Hepatocytes to Interferon-α in Chimeric MiceBackground & AimsInterferon (IFN)-α therapy is not effective for most patients with chronic hepatitis B virus (HBV) infection for reasons that are not clear. We investigated whether HBV infection reduced IFN-α–mediated induction of antiviral defense mechanisms in human hepatocytes.
MethodsHuman hepatocytes were injected into severe combined immune-deficient mice (SCID/beige) that expressed transgenic urokinase plasminogen activator under control of the albumin promoter. Some mice were infected with HBV; infected and uninfected mice were given injections of human IFN-α. Changes in viral DNA and expression of human interferon-stimulated genes (ISGs) were measured by real-time polymerase chain reaction, using human-specific primers, and by immunohistochemistry.
ResultsMedian HBV viremia (0.8log) and intrahepatic loads of HBV RNA decreased 3-fold by 8 or 12 hours after each injection of IFN-α, but increased within 24 hours. IFN-α activated expression of human ISGs and nuclear translocation of signal transducers and activators of transcription–1 (STAT1) in human hepatocytes that repopulated the livers of uninfected mice. Although baseline levels of human ISGs were slightly increased in HBV-infected mice, compared with uninfected mice, IFN-α failed to increase expression of the ISGs OAS-1, MxA, MyD88, and TAP-1 (which regulates antigen presentation) in HBV-infected mice. IFN-α did not induce nuclear translocation of STAT1 in HBV-infected human hepatocytes. Administration of the nucleoside analogue entecavir (for 20 days) suppressed HBV replication but did not restore responsiveness to IFN-α.
ConclusionsHBV prevents induction of IFN-α signaling by inhibiting nuclear translocation of STAT1; this can interfere with transcription of ISGs in human hepatocytes. These effects of HBV might contribute to the limited effectiveness of endogenous and therapeutic IFN-α in patients and promote viral persistence.
Keywords: cccDNA, uPA, Antiviral Therapy, Innate Immunity Abbreviations used in this paper: cccDNA, covalently closed circular DNA, ETV, entecavir, IFN, interferon, ISGs, interferon-stimulated genes, MHC, major histocompatibility complex, MxA, mixovirus resistance protein A, OAS-1, 2′-5′ oligoadenylate synthase 1, PCR, polymerase chain reaction, pgRNA, pregenomic RNA, rcDNA, relaxed circular DNA, SCID, severe combined immune-deficient, STAT, signal transducers and activators of transcription, TLR, Toll-like receptor, uPA, urokinase plasminogen activator |