[英语，研究]Changes of cytotoxic T lymphocytes (CTLs), natural kill

StephenW

http://7thspace.com/headlines/380999/dynamic_changes_of_cytotoxic_t_lymphocytes_ctls_natural_killer_nk_cells_and_natural_killer_t_nkt_cells_in_patients_with_acute_hepatitis_b_infection.html
The goal of this study is to observe changes in HBcAg-specific cytotoxic T lymphocytes (CTLs), natural killer (NK) and natural killer T (NKT) cells from peripheral blood and to relate such changes on viral clearance and liver injury in patients with acute hepatitis B (AHB).

Methods: Dynamic profiles on the frequency of HLA-A0201-restricted HBcAg18-27 pentamer complex (MHC-Pentamer)-specific CTLs and lymphocyte subsets in AHB patients were analyzed in addition to liver function tests, HBV serological markers, and HBV DNA levels.  ELISPOT was used to detect interferon-gamma (INF-gamma) secretion in specific CTLs stimulated with known T cell epitope peptides associated with HBV surface protein, polymerase, and core protein.

Results: HBV-specific CTL frequencies in AHB patients were much higher than in patients with chronic hepatitis B (CHB) (p <0.05).

  HBeAg and HBV DNA disappeared earlier in AHB patients with a high frequency of HBV-specific CTLs compared with those with a low frequency of HBV-specific CTLs (p = 0.001 and 0.024, respectively).  INF-gamma spots of effector cells stimulated by Pol575-583, Env348-357, or Core18-27 epitope peptides were significantly greater in AHB patients than in CHB patients (p <0.01).

  CD3+CD8+ T cell numbers in AHB patients was more than observed in the healthy control group from the first to the fourth week after admission (p = 0.008 and 0.01, respectively); the number of CD3+CD8+ T cells and frequency of HBcAg18-27-specific CTLs in AHB patients reached peak levels at the second week after admission. NK and NKT cell numbers were negatively correlated with the frequency of HBcAg-specific CTLs (r = -0.266, p = 0.05).

Conclusions: Patients with AHB possess a higher frequency of HBcAg-specific CTLs than CHB patients.

The frequency of specific CTLs in AHB patients is correlated with HBeAg clearance indicating that HBV-specific CTLs play an important role in viral clearance and the self-limited process of the disease. Furthermore, NK and NKT cells are likely involved in the early, non-specific immune response to clear the virus.

Author: Jun LiYaping HanKe JinYufeng WanShixia WangBo LiuYuan LiuShan LuZuhu Huang
Credits/Source: Virology Journal 2011, 8:199



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StephenW

这项研究的目的是观察HBcAg特异性细胞毒性T淋巴细胞的变化（淋巴细胞），自然杀伤（NK）和自然杀伤T（NKT细胞）细胞和外周血这种变化涉及患者对病毒清除，肝损伤急性乙型肝炎（AHB）的。

方法：对HLA - A020​​1 -限制HBcAg18 - 27五聚体复合体（MHC -五聚体）频率动态特征文件，特异性CTL和AHB患者淋巴细胞亚群进行了分析，除了肝功能检查，乙肝病毒血清学标志和HBV DNA水平。酶联免疫斑点法检测干扰素-γ（干扰素-γ）与已知的T细胞表位肽与乙肝病毒表面蛋白，聚合酶，并刺激相关的核心蛋白特异性CTL分泌。

结果：AHB患者HBV特异性CTL的频率与慢性乙型肝炎患者要比较高（乙肝）性（P“0.05）。

HBeAg和HBV DNA的消失早，一对HBV特异性CTL的高频率相比，与一对HBV特异性CTL的低频率（P = 0.001和0.024，分别）那些在AHB患者。干扰素-γ名胜刺激效应细胞Pol575 - 583，Env348 - 357，或Core18 - 27表位肽AHB患者显着高于慢性乙型肝炎患者（P <0.01）。

外周血CD3 + CD8 +的AHB的患者T细胞数量较观察健康对照组从第一次住院后的第四周（p = 0.008和0.01），而数量的CD3 + CD8 + T细胞和HBcAg18频在AHB患者27特异性CTL达到入院后第二周最高水平。 NK细胞和NKT细胞数量呈负相关，与HBcAg特异性CTL的频率（r = -0.266，p值= 0.05）。

结论：与AHB患者拥有一个比慢性乙型肝炎患者HBcAg特异性CTL的频率较高。

在AHB患者特异性CTL频率与HBeAg的间隙，表明乙肝病毒特异性CTL发挥在病毒清除和疾病的自限性过程中的重要作用密切相关。此外，NK细胞和NKT细胞有可能参与了早期，非特异性免疫反应以清除病毒。

作者：六月LiYaping汉克JinYufeng WanShixia WangBo柳园黄刘善LuZuhu
学分/来源：病毒学杂志2011，8:199

lin12345

这个我喜欢 都说乙肝是免疫类疾病 长久 免疫控制才是王道