Hepatitis B virus genotyping: current methods and clinical implications

liver411

<http://www.ijidonline.com/articl ... 39/abstract?rss=yes>

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
Articles in Press

Hepatitis B virus genotyping: current methods and clinical implications

Bassem S.S. Guirgisa, Radwa O. Abbasb, Hassan M.E. Azzazyab

Received 26 December 2008; received in revised form 10 March 2010; accepted 11
March 2010. published online 02 August 2010.
Corrected Proof

Summary
Hepatitis B virus (HBV) is a major cause of liver cirrhosis and hepatocellular
carcinoma (HCC). Eight different HBV genotypes have been identified with
distinct geographical distributions. Different genotyping methods exist
including sequencing, INNO-LiPA, restriction fragment polymorphism (RFLP),
multiplex PCR, serotyping, oligonucleotide microarray chips, reverse dot blot,
restriction fragment mass polymorphism (RFMP), invader assay, and real-time
PCR. Several investigators have studied the influence of HBV genotypes on
clinical outcomes in chronic HBV patients. This review describes the current
genotyping techniques, as well as their advantages and limitations. It also
presents the clinical evidence that correlates HBV genotypes to clinical
outcomes including disease severity, HCC development, response to therapy,
disease chronicity, transplantation outcomes, and occult infection.

Corresponding Editor: Mark Holodniy, California, USA

a Yousef Jameel Science and Technology Research Center, The American
University in Cairo, New Cairo, Egypt
b Department of Chemistry, The American University in Cairo, AUC Avenue, PO
Box 74, New Cairo 11835, Egypt

Corresponding author. Tel.: +20 2 2615 2543; fax: +20 2 2795 7565.

PII: S1201-9712(10)02423-9
doi:10.1016/j.ijid.2010.03.020
© 2010 International Society for Infectious Diseases. Published by Elsevier
Inc. All rights reserved



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