乙型肝炎病毒表面蛋白在调节完整和不完整病毒体成熟和分

StephenW

乙型肝炎病毒表面蛋白在调节完整和不完整病毒体成熟和分泌中的作用
徐明珠 1 , 常丽 1 , 丁家辉 2 , 吴敏 1 , 汤义杰 1 , 袁正宏 2 , 张晓楠 1 3
隶属关系
隶属关系

    1
    复旦大学上海公共卫生临床中心，中国上海。
    2
    复旦大学上海医学院医学分子病毒学重点实验室，上海，中国。
    3
    澳大利亚首都堪培拉大学科技学院治疗解决方案研究中心。

    PMID：35438623 DOI：10.1099/jgv.0.001733

抽象的

各种形式的乙型肝炎病毒（HBV）表面蛋白的表达调节成熟病毒粒子的释放，但它们是否影响其他不完整病毒颗粒的释放，如裸衣壳，尚不清楚。在这里，通过在 HepAD38 细胞中稳定过表达大或中/小乙型肝炎表面蛋白（LHBs、M/SHBs），我们评估了它们对完整和不完整病毒颗粒释放的影响。 LHBs 的过度生产抑制了所有表面蛋白的释放，这增加了裸衣壳/病毒粒子的比例。这种效应伴随着升高的细胞外HBV RNA。另一方面，M/SHBs 的过表达大大提高了包膜病毒和亚病毒颗粒的分泌。当 LHBs 过表达时，病毒 DNA 和 LHBs 的原位可视化揭示了成熟病毒粒子的细胞内保留。这些结果表明，有包膜或无包膜病毒颗粒分泌的分子决定受细胞内大、中、小表面抗原的比例调节。这种机制可能与 HBV 诱发的慢性肝病的进展和消退有关。

关键词：HBV RNA； HBV病毒体； LHB； SHB；裸衣壳。

StephenW

The role of hepatitis B virus surface proteins in regulating the maturation and secretion of complete and incomplete virions
Mingzhu Xu  1 , Chang Li  1 , Jiahui Ding  2 , Min Wu  1 , Yijie Tang  1 , Zhenghong Yuan  2 , Xiaonan Zhang  1   3
Affiliations
Affiliations

    1
    Shanghai Public Health Clinical Center, Fudan University, Shanghai, PR China.
    2
    Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, PR China.
    3
    Centre for Research in Therapeutic Solutions, Faculty of Science and Technology, University of Canberra, ACT, Australia.

    PMID: 35438623 DOI: 10.1099/jgv.0.001733

Abstract

The expression of various forms of hepatitis B virus (HBV) surface proteins regulates the release of mature virion, but whether they affect the release of other incomplete viral particles, such as naked capsid, is not clear. Here, by stable overexpression of large or middle/small hepatitis B surface proteins (LHBs, M/SHBs) in HepAD38 cells, we evaluated their effects on the release of complete and incomplete viral particles. Overproduction of LHBs inhibited the release of all surface proteins, which increased the ratio of naked capsids/virions. This effect was accompanied by the elevated extracellular HBV RNA. On the other hand, overexpression of M/SHBs greatly improved the secretion of enveloped viral and subviral particles. In situ visualization of viral DNA and LHBs revealed intracellular retention of mature virions when LHBs were overexpressed. These results indicate that the molecular decision on secretion of enveloped or unenveloped viral particles is modulated by the intracellular ratio of large, middle and small surface antigens. This mechanism may be relevant in the progression and resolution of HBV-induced chronic liver disease.

Keywords: HBV RNA; HBV virions; LHBs; SHBs; naked capsids.