EASL2015:在大型定量成分的差异， 中小乙型肝炎病毒（HBV）表

StephenW

P0561
DIFFERENCES IN QUANTITATIVE COMPOSITION OF LARGE,
MIDDLE AND SMALL HEPATITIS B VIRUS (HBV) SURFACE
ANTIGEN (HBsAg) IN ACUTE AND CHRONIC HBV INFECTION
M. Grossmann1, S. B¨ohm1, D. Glebe2, T. Berg1, F. van B¨ommel1.
1Division of Gastroenterology and Hepatology, University Hospital
Leipzig, Leipzig, 2Institute for Medical Virology, University Hospital
Giessen, Giessen, Germany
E-mail: [email protected]
Background and Aims: Quantification of HBsAg has been used as
a marker to predict the course of HBV infections and response
to antiviral treatment. The viral surface antigen consists of three
components, large (L-), middle (M-) and small (S-) HBsAg, which
differ in amino-terminal sequences and glycosylation status. In
different ratios, all HBsAg components are part of infectious
virions and non-infectious subviral particles. Quantitative analysis
of HBsAg composition in serum can be a potential marker to
assess the relative proportion of infectious virions. Additionally,
there are evidences indicating that the L-HBsAg plays an important
role in viral entry of hepatocytes. However, the clinical value of
determining HBsAg components has not been established yet.
In this study, we established a sensitive ELISA for quantitative
detection of the different HBsAg components and determination
of the HBsAg composition in patients with either acute or chronic
HBV infections.
Methods: Microtiter plates were coated with specific antibodies
and blocked with different reagents to optimize the test. L-, M- and
S-HBsAg were quantified in serum samples of 23 patients (11 acute
and 12 inactive chronic HBV infections) without antiviral therapy
and a wide range of known HBsAg in total (mean 14.490 IU/ml,
range 1.68–124,000 IU/ml) measured by a commercial assay (Abbott
Architect). Three healthy patients served as negative control.
Results: Detection of lowest quantities (2–40 ng/ml) of all
three HBsAg components was possible. The mean serum levels
of HBsAg in patients with acute and chronic HBV infection
were 4.81±4.82 log10 ng/ml (range, 0.67 log10 to 5.47 log10 ng/ml)
and 4.31±4.20 log10 ng/ml (range, 1.63 log10 to 4.76 log10 ng/ml;
p = 0.128). The mean ratios of L-, M- and S-HBsAg in patients
with acute and chronic HBV infections were 25% versus 17%
(p = 0.073), 8% versus 4% (p = 0.016) and 66% versus 78% (p = 0.021),
respectively.
Conclusions: Different phases of HBV infection may be
characterized by different ratios in HBsAg components. Quantitative
analysis of HBsAg composition in serum can be a potential marker
to characterize the course of HBV infections and the response to
antiviral treatment.

StephenW

P0561
在大型定量成分的差异，
中小乙型肝炎病毒（HBV）表面
抗原（HBsAg）急性期和慢性HBV感染
M. Grossmann1，SB¨ohm1，D Glebe2，T. Berg1，F面包车B¨ommel1。
胃肠病学和肝病，大学附属医院1区
莱比锡，莱比锡，为2中国科学院医学病毒学，大学附属医院
吉森，德国吉森
电子信箱：[email protected]
背景和目的：乙肝表面抗原定量已被用作
一个标记，以预测的HBV感染和响应的过程中
抗病毒治疗。病毒表面抗原包括三个
部件，大型（L-），中（M-）和小（S-）的HBsAg，这
不同的氨基末端序列和糖基化状态。在
不同的比例，所有的乙肝表面抗原成分是传染性组成部分
病毒粒子和非感染性亚病毒颗粒。定量分析
HBsAg的组合物的血清中可以是一个潜在的标记，以
评估感染性病毒体的相对比例。此外，
有证据表明L- HBsAg的起着重要
在肝细胞中的病毒进入角色。但是，临床应用价值
确定HBsAg的部件已尚未建立。
在这项研究中，我们建立了一个敏感的ELISA定量
检测乙肝表面抗原的不同组件和决心
患者急性或慢性乙肝表面抗原组合物的
HBV感染。
方法：微量滴定板用特异性抗体
并且用不同的试剂来优化测试。 L-，M-和
S-的HBsAg定量的23例患者的血清样本中（急性11
12非活动慢性HBV感染）没有抗病毒治疗
而在总的范围广泛的已知乙肝表面抗原（平均14.490 IU / ml时，
范围1.68-124,000 IU / ml）的由商业法检测（雅培
建筑师）。三患者的健康作为阴性对照。
结果：最低检测量都（2-40纳克/毫升）的
3 HBsAg的组件是可能的。平均血清水平
乙肝表面抗原在急性和慢性HBV感染的
分别为4.81±4.82日志10纳克/毫升（范围，0.67日志10 5.47日志10纳克/毫升）
和4.31±4.20日志10纳克/毫升（范围，1.63日志10 4.76日志10纳克/毫升;
P = 0.128）。的平均比率L-，M-和S-HBsAg的患者
急性和慢性HBV感染分别为25％和17％
（p值= 0.073），8％和4％（p值= 0.016）和66％对78％（p值= 0.021），
分别。
结论：HBV感染的不同阶段可能
特点是乙肝表面抗原成分比例不同。量
在血清HBsAg组合物的分析可以是一个潜在的标记
对HBV感染的过程中，响应来表征
抗病毒治疗。

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HBV感染不同阶段，表面抗原组成成分比例不同？l表面抗原，M表面抗原，S表面抗原在急满慢性感染者的表面抗原血清组成比例不同。