多块外植体肝组织中病毒 RNA 的定量显示在乙型肝炎感染中存

StephenW

多块外植体肝组织中病毒 RNA 的定量显示在乙型肝炎感染中存在明显的局灶性差异
Gustaf E Rydell、Kasthuri Prakash、Simon B Larsson、Catarina Skoglund、Johan Ringlander、Maria E Andersson、Maria Castedal、Heléne Norder、Magnus Lindh
传染病杂志，第 226 卷，第 6 期，2022 年 9 月 15 日，第 1036–1040 页，https://doi.org/10.1093/infdis/jiab469
发表：
2021 年 9 月 17 日
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抽象的

乙型肝炎病毒 (HBV) DNA 和 RNA 通过数字 PCR 测定对来自 4 个由 HBV 引起的肝硬化的肝外植体中的每一个的 20-30 个组织块进行定量。不同片段之间 HBV RNA 水平的患者体内变异性高达 1000 倍。当复制高时，核心 RNA 和 S RNA 水平相似且相关性很强，这支持转录来自共价闭合环状 DNA (cccDNA)。相比之下，在中高或低复制的患者中，S RNA 相对于 cccDNA 和核心 RNA 的表达增强支持了 HBV 表面抗原（HBsAg）在此类患者中主要由整合的 HBV DNA 表达。
液滴数字 PCR, 乙型肝炎病毒, HBsAg, 肝硬化, 肝细胞癌
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简短报告

StephenW

Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection
Gustaf E Rydell, Kasthuri Prakash, Simon B Larsson, Catarina Skoglund, Johan Ringlander, Maria E Andersson, Maria Castedal, Heléne Norder, Magnus Lindh Author Notes
The Journal of Infectious Diseases, Volume 226, Issue 6, 15 September 2022, Pages 1036–1040, https://doi.org/10.1093/infdis/jiab469
Published:
17 September 2021
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Abstract

Hepatitis B virus (HBV) DNA and RNA were quantified by digital PCR assays in 20–30 tissue pieces from each of 4 liver explants with cirrhosis caused by HBV. The within-patient variability of HBV RNA levels between pieces was up to a 1000-fold. Core RNA and S RNA levels were similar and correlated strongly when replication was high, supporting that transcription was from covalently closed circular DNA (cccDNA). By contrast, enhanced expression of S RNA relative to cccDNA and core RNA in patients with medium-high or low replication supports that HBV surface antigen (HBsAg) can be expressed mainly from integrated HBV DNA in such patients.
droplet digital PCR, hepatitis B virus, HBsAg, cirrhosis, hepatocellular carcinoma
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Brief Report

StephenW

https://doi.org/10.1093/infdis/jiab469