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Expression of Functional Molecule on Plasmacytoid Dendritic Cells Is Associated With HBsAg Loss in HBeAg-Positive Patients During PEG-IFN α-2a Treatment
Weihua Cao 1 2 , Si Xie 3 , Lu Zhang 1 , Xiaoyue Bi 1 , Yanjie Lin 4 , Liu Yang 1 , Yao Lu 1 , Ruyu Liu 1 , Min Chang 1 , Shuling Wu 1 , Ge Shen 1 , Jianping Dong 5 , Yao Xie 1 4 , Minghui Li 1 4
Affiliations
Affiliations
1
Department of Hepatology Division 2, Beijing Ditan Hospital, Capital Medical University, Beijing, China.
2
Department of Infectious Diseases, Miyun Teaching Hospital, Capital Medical University, Beijing, China.
3
Division of Hepatology, Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.
4
Department of Hepatology Division 2, Peking University Ditan Teaching Hospital, Beijing, China.
5
Department of Infectious Diseases, Haidian Hospital, Beijing Haidian Section of Peking University Third Hospital, Beijing, China.
PMID: 35663955 PMCID: PMC9160736 DOI: 10.3389/fimmu.2022.891424
Abstract
Objective: The ideal endpoint of antiviral therapy in chronic hepatitis B (CHB) patients is to clear hepatitis B surface antigen (HBsAg). This study aimed to evaluate whether the expression of functional molecules on plasmacytoid dendritic cells (pDCs) is associated with HBsAg loss in HBeAg-positive patients during peginterferon alpha-2a (PEG IFN α-2a) therapy.
Methods: A single-center prospective cohort study was performed in HBeAg-positive CHB patients who were treated with PEG-IFN α-2a and followed up for 4 years. HBsAg clearance, HBeAg loss and undetectable HBV DNA achieved by PEG-IFN α-2a therapy was considered as functional cure. The frequencies of pDC and CD86+ pDC in peripheral blood, and the mean fluorescence intensity of CD86 (CD86MFI) on the surface of pDC were measured at starting therapy, after 12 and 24 weeks of therapy.
Results: Of 63 patients enrolled, 17 patients achieved HBsAg loss. The baseline HBV DNA load in Non-functional-cure group was significantly higher than that in Functional cure group, and the CD86+ pDC% was significantly lower in patients without functional cure. HBV DNA load (OR=0.146, P = 0.002) and CD86+ pDC% (OR=1.183, P = 0.025) were independent factors associated with functional cure confirmed by binary logistic regression analysis. In the Functional cure group, HBsAg, HBeAg, and HBV DNA loads decreased remarkably after 12 weeks and 24 weeks of treatment compared to baseline. In Non-functional-cure group, CD86+ pDC% and CD86MFI increased significantly from baseline after 12 weeks of treatment. In the Functional cure group, compared with baseline, pDC% increased significantly at 24 weeks, while CD86MFI increased significantly after 24 weeks of treatment.
Conclusion: The lower the baseline HBV DNA load and the more the baseline CD86+ pDC%, the easier it is for patients to obtain functional cure.
Keywords: chronic hepatitis B; functional cure; hepatitis B surface antigen; interferon; plasmacytoid dendritic cells.
Copyright © 2022 Cao, Xie, Zhang, Bi, Lin, Yang, Lu, Liu, Chang, Wu, Shen, Dong, Xie and Li.
Conflict of interest statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The Reviewer LJ declared a shared parent affiliation with the authors WC, LZ, XB, YL, LY, YJL, RL, MC, SW, GS, YX, and ML to the handling editor at the time of review.
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