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标题: 重新审视乙型肝炎血清转化：从定量和高度敏感的测定法和 [打印本页]

作者: StephenW 时间: 2021-11-30 19:03 标题: 重新审视乙型肝炎血清转化：从定量和高度敏感的测定法和

重新审视乙型肝炎血清转化：从定量和高度敏感的测定法和新型生物标志物对急性乙型肝炎病毒 (HBV) 感染自然史的新见解

Mary C. Kuhns、Vera Holzmayer、Anne L. McNamara、Mark Anderson 和 Gavin A. Cloherty

病毒学杂志第 18 卷，文章编号：235 (2021) 引用此文章

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抽象的
背景

典型急性自限性感染期间的乙型肝炎病毒 (HBV) 血清标志物通常被描述为传统 HBV 标志物的复合物。目前的研究更新并扩展了我们对急性乙型肝炎的认识，对来自五名急性 HBV 血浆置换供体的纵向样本的定量分子和血清学数据进行了更新。
方法

对来自 5 名急性 HBV 血浆置换捐献者的 137 份纵向样本进行了检测，其中 4 名具有自限性感染，1 名发生持续感染。检测包括定量乙型肝炎表面抗原 (HBsAg)、HBV 抗原抗体、定量 HBV e 抗原 (HBeAg)、HBV DNA、定量 HBV 核心相关抗原 (HBcrAg)、高度敏感的 ARCHITECT HBsAg NEXT (HBsAgNx) 检测，以及HBV 前基因组 RNA (pg RNA) 的定量研究分析。
结果

HBV DNA 和 HBsAg 的峰值水平在各组之间相差几个数量级（HBV DNA 为 2.2 × 105–2.7 × 109 IU/ml，HBsAg 为 7.9-1.1 × 105 IU/ml）。 HBsAg 水平在 HBV DNA 峰值后平均 2.8 天达到峰值。与四个面板中的定量测定相比，更灵敏的 HBsAgNx 测定增加了观察到的 HBsAg 阳性的总体持续时间。在三个小组中 HBsAg 消失后观察到间歇性可检测的低水平 HBV DNA。 HBeAg 水平峰值出现在 DNA 峰值后 2-20 天，范围为 1.1 至 4.5 × 103 IU/ml。在感染消退的四个面板中，在 HBV DNA 峰值后 19-317 天检测到表明免疫的抗 HBs 水平（≥ 10 mIU/ml）。最大 HBcrAg 浓度范围为 1 × 105 至 > 6.4 × 106 U/ml，并与 HBeAg 值（R2 = 0.9495）和 HBV DNA 值（R2 = 0.8828）相关。峰值 pgRNA 值范围从 1.6 × 103 到 1.4 × 108 U/ml，并与 HBV DNA 相关（R2 = 0.9013）。
结论

传统的和新的/新的 HBV 生物标志物被用于生成跨越急性 HBV 早期到晚期阶段的血清转换组的分子和血清学特征。血清转化曲线是异质的，可能有助于了解相对于典型复合代表的急性曲线谱。

作者: StephenW 时间: 2021-11-30 19:04

Hepatitis B seroconversion revisited: new insights into the natural history of acute hepatitis B virus (HBV) infection from quantitative and highly sensitive assays and novel biomarkers

Mary C. Kuhns, Vera Holzmayer, Anne L. McNamara, Mark Anderson & Gavin A. Cloherty

Virology Journal volume 18, Article number: 235 (2021) Cite this article

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Abstract
Background

Hepatitis B virus (HBV) serum markers during typical acute self-limited infection are usually depicted as a composite of traditional HBV markers. The current study updates and expands our knowledge of acute hepatitis B with quantitative molecular and serological data on longitudinal samples from five plasmapheresis donors with acute HBV.
Methods

137 longitudinal samples from five plasmapheresis donors with acute HBV were tested, four with self-limited infection and one who developed persistent infection. Testing included quantitative hepatitis B surface antigen (HBsAg), antibodies to HBV antigens, quantitative HBV e antigen (HBeAg), HBV DNA, quantitative HBV core-related antigen (HBcrAg), the highly sensitive ARCHITECT HBsAg NEXT (HBsAgNx) assay, and a quantitative research assay for HBV pregenomic RNA (pg RNA).
Results

Peak levels of HBV DNA and HBsAg differed by several orders of magnitude among the panels (2.2 × 105–2.7 × 109 IU/ml for HBV DNA and 7.9–1.1 × 105 IU/ml for HBsAg). HBsAg levels peaked an average of 2.8 days after the HBV DNA peak. The overall duration of observed HBsAg positivity was increased by the more sensitive HBsAgNx assay compared to the quantitative assay in four panels. Intermittently detectable low-level HBV DNA was observed after HBsAg loss in three panels. Peak HBeAg levels occurred 2–20 days after the DNA peak and ranged from 1.1 to 4.5 × 103 IU/ml. In four panels with resolution of infection, anti-HBs levels indicating immunity (≥ 10 mIU/ml) were detected 19–317 days after the HBV DNA peak. Maximum HBcrAg concentrations ranged from 1 × 105 to > 6.4 × 106 U/ml and correlated with HBeAg values (R2 = 0.9495) and with HBV DNA values (R2 = 0.8828). Peak pgRNA values ranged from 1.6 × 103 to 1.4 × 108 U/ml and correlated with HBV DNA (R2 = 0.9013).
Conclusion

Traditional and new/novel HBV biomarkers were used to generate molecular and serological profiles for seroconversion panels spanning the early to late phases of acute HBV. Seroconversion profiles were heterogeneous and may be instructive in appreciating the spectrum of acute profiles relative to the typical composite representation.

作者: StephenW 时间: 2021-11-30 19:04

https://virologyj.biomedcentral. ... /s12985-021-01706-w

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