通过超高效液相色谱/串联质谱法快速定量乙型肝炎单次感染

StephenW

Rapid Commun Mass Spectrom. 2020 Jan 20:e8728. doi: 10.1002/rcm.8728. [Epub ahead of print]
Rapid quantification of tenofovir in umbilical cord plasma and amniotic fluid in hepatitis B mono-infected pregnant women during labor by Ultra Performance Liquid Chromatography/Tandem Mass Spectrometry.
Ma Z1, Li S1, He D1, Wang Y1, Jiang H2, Zhou H2, Jin J1, Lin N1.
Author information

1
    Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
2
    Laboratory of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, P.R. China.

Abstract
RATIONALE:

Tenofovir (TFV) is a first-line antiviral agent against hepatitis B virus (HBV) and is recommended for the prevention of mother-to-infant transmission of HBV. To study the distribution of TFV in umbilical cord plasma and amniotic fluid of HBV-infected pregnant women, a rapid and sensitive method for TFV determination was developed and validated.
METHODS:

The quantification method was developed using liquid chromatography coupled to tandem mass spectrometry. The analytes were separated on an ACQUITY UPLC HSS T3 column under gradient elution with methanol and 0.01% ammonia solution in 10 mM ammonium acetate-water. This is the first reported method for the determination of TFV using alkaline rather than acidic mobile phases. Linearity, accuracy, precision, limit of quantification, specificity and stability were assessed.
RESULTS:

Detection of TFV was achieved within 4 min. The calibration curves for TFV quantification showed excellent linearity in the range of 1-500 ng/mL. The intra- and interbatch precision and accuracy ranged from -4.35% to 6.92%. This method was successfully applied to determination of samples from 50 HBV mono-infected women undergoing tenofovir disoproxil fumarate therapy. The mean concentrations of TFV in the umbilical cord and amniotic fluid samples were 29.2 (4.6-86) and 470.9 (156-902) ng/mL, respectively, which showed a moderate positive correlation (r= 0.5299, P<0.001).
CONCLUSIONS:

A simple, rapid but sensitive bioanalytical method to determine TFV concentration in both umbilical cord plasma and amniotic fluid using LC/MS/MS was developed and applied to HBV-infected women during labor who were undergoing TDF therapy, which will help us understand the efficacy and safety of tenofovir during pregnancy.

This article is protected by copyright. All rights reserved.

PMID:
    31960519
DOI:
    10.1002/rcm.8728

StephenW

快速通讯质谱。 2020年1月20日：e8728。 doi：10.1002 / rcm.8728。 [Epub提前发行]
通过超高效液相色谱/串联质谱法快速定量乙型肝炎单次感染孕妇在分娩过程中脐带血浆和羊水中的替诺福韦。
马Z1，李S1，何D1，王Y1，江H2，周H2，金J1，林N1。
作者信息

1个
    浙江大学医学院附属杭州市第一人民医院，浙江杭州
2
    浙江大学药学院药物分析与药物代谢实验室，浙江杭州

抽象
理由：

替诺福韦（TFV）是抗乙型肝炎病毒（HBV）的一线抗病毒药物，建议用于预防母婴传播HBV。为了研究TFV在HBV感染孕妇的脐带血浆和羊水中的分布，开发并验证了一种快速灵敏的TFV测定方法。
方法：

定量方法是使用液相色谱与串联质谱联用开发的。在ACQUITY UPLC HSS T3色谱柱上，在10 mM乙酸铵-水中的甲醇和0.01％氨溶液进行梯度洗脱的条件下，分离分析物。这是首次报道的使用碱性而非酸性流动相测定TFV的方法。评估了线性，准确性，精密度，定量限，特异性和稳定性。
结果：

在4分钟内检测到TFV。 TFV定量的校准曲线在1-500 ng / mL的范围内显示出极好的线性。批内和批间的精度和准确度范围为-4.35％至6.92％。该方法已成功地用于测定接受替诺福韦酯二富马酸富马酸酯治疗的50例HBV单一感染妇女的样品。脐带和羊水样本中TFV的平均浓度分别为29.2（4.6-86）和470.9（156-902）ng / mL，显示出中等正相关（r = 0.5299，P <0.001）。
结论：

开发了一种简单，快速但灵敏的生物分析方法，使用LC / MS / MS测定脐带血浆和羊水中的TFV浓度，并将其应用于正在接受TDF治疗的分娩过程中被HBV感染的妇女，这将有助于我们了解疗效和替诺福韦在怀孕期间的安全性。

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PMID：
    31960519
DOI：
    10.1002 / rcm.8728