15/10/02说明:此前论坛服务器频繁出错,现已更换服务器。今后论坛继续数据库备份,不备份上传附件。

肝胆相照论坛

 

 

肝胆相照论坛 论坛 学术讨论& HBV English 鉴定和表征乙型肝炎病毒X蛋白和Smc5 / 6之间的相互作用 ...
查看: 522|回复: 2
go

鉴定和表征乙型肝炎病毒X蛋白和Smc5 / 6之间的相互作用 克里

Rank: 8Rank: 8

现金
62111 元 
精华
26 
帖子
30441 
注册时间
2009-10-5 
最后登录
2022-12-28 

才高八斗

发表于 2017-4-26 23:14 |显示全部帖子
Viruses 2017, 9(4), 69; doi:10.3390/v9040069
Identifying and Characterizing Interplay between Hepatitis B Virus X Protein and Smc5/6
Christine M. Livingston 1
, Dhivya Ramakrishnan 1
, Michel Strubin 2
, Simon P. Fletcher 1
and Rudolf K. Beran 1,*
1Gilead Sciences, Foster City, CA 94404, USA
2Department of Microbiology and Molecular Medicine, University Medical Center (CMU), 1211 Geneva, Switzerland
*
Author to whom correspondence should be addressed.
Received: 28 February 2017 / Revised: 27 March 2017 / Accepted: 29 March 2017 / Published: 3 April 2017

Abstract
Hepatitis B X protein (HBx) plays an essential role in the hepatitis B virus (HBV) replication cycle, but the function of HBx has been elusive until recently. It was recently shown that transcription from the HBV genome (covalently-closed circular DNA, cccDNA) is inhibited by the structural maintenance of chromosome 5/6 complex (Smc5/6), and that a key function of HBx is to redirect the DNA-damage binding protein 1 (DDB1) E3 ubiquitin ligase to target this complex for degradation. By doing so, HBx alleviates transcriptional repression by Smc5/6 and stimulates HBV gene expression. In this review, we discuss in detail how the interplay between HBx and Smc5/6 was identified and characterized. We also discuss what is known regarding the repression of cccDNA transcription by Smc5/6, the timing of HBx expression, and the potential role of HBx in promoting hepatocellular carcinoma (HCC). View Full-Text
Keywords: HBx; HBV; DDB1; Smc5/6; cccDNA

Rank: 8Rank: 8

现金
62111 元 
精华
26 
帖子
30441 
注册时间
2009-10-5 
最后登录
2022-12-28 

才高八斗

发表于 2017-4-26 23:14 |显示全部帖子
病毒2017,9(4),69; doi:10.3390 / v9040069
鉴定和表征乙型肝炎病毒X蛋白和Smc5 / 6之间的相互作用
克里斯汀·利文斯顿1
,Dhivya Ramakrishnan 1
,Michel Strubin 2
,Simon P. Fletcher 1
和Rudolf K.Banan 1,*
1 Gilead Sciences,Foster City,CA 94404,USA
2,瑞士日内瓦大学医学中心微生物与分子医学系,1211
*
作者应向谁传递信函。
收到日期:2017年2月28日/修订日期:2017年3月27日/接受日期:2017年3月29日/发布日期:2017年4月3日

抽象
乙型肝炎X蛋白(HBx)在乙型肝炎病毒(HBV)复制周期中起着至关重要的作用,但HBx的功能直到最近都是难以捉摸的。最近显示,通过染色体5/6复合物(Smc5 / 6)的结构维持抑制了HBV基因组的转录(共价闭合的环状DNA,cccDNA),HBx的关键功能是将DNA-损伤结合蛋白1(DDB1)E3泛素连接酶靶向该复合物进行降解。通过这样做,HBx缓解Smc5 / 6的转录抑制并刺激HBV基因表达。在这次审查中,我们详细讨论了HBx和Smc5 / 6之间的相互作用如何被识别和表征。我们还讨论了Smc5 / 6抑制cccDNA转录,HBx表达的时机以及HBx在促进肝细胞癌(HCC)中的潜在作用。查看全文
关键词:HBx; HBV; DDB1; Smc5 / 6; cccDNA

Rank: 8Rank: 8

现金
62111 元 
精华
26 
帖子
30441 
注册时间
2009-10-5 
最后登录
2022-12-28 

才高八斗

发表于 2017-4-26 23:14 |显示全部帖子
你需要登录后才可以回帖 登录 | 注册

肝胆相照论坛

GMT+8, 2024-3-29 20:39 , Processed in 0.013142 second(s), 11 queries , Gzip On.

Powered by Discuz! X1.5

© 2001-2010 Comsenz Inc.