EASL2015:REP 2139发现，以减少乙肝表面抗原在白种人与HBV / HDV合

StephenW

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RNA病毒在细胞内的复制，是不依赖与病毒释放的正确. 但复制后不能释放, 永远存在胞浆内?

StephenW

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核糖核酸酶（英语：ribonuclease，常用縮寫：RNase）或稱RNA酶，是一種可將RNA水解成小分子組成的核酸酶（nuclease）。可粗分為核糖核酸內切酶（endoribonuclease）與核糖核酸外切酶（exoribonuclease），這些酶分別歸屬於EC 2.7（磷酸化酶）與EC 3.1（水解酶）中的多個次分類。

All organisms studied contain many RNases of many different classes, showing that RNA degradation is a very ancient and important process. As well as cleaning of cellular RNA that is no longer required, RNases play key roles in the maturation of all RNA molecules, both messenger RNAs that carry genetic material for making proteins, and non-coding RNAs that function in varied cellular processes. In addition, active RNA degradation systems are a first defense against RNA viruses, and provide the underlying machinery for more advanced cellular immune strategies such as RNAi.

Some cells also secrete copious quantities of non-specific RNases such as A and T1. RNases are, therefore, extremely common, resulting in very short lifespans for any RNA that is not in a protected environment. It is worth noting that all intracellular RNAs are protected from RNase activity by a number of strategies including 5' end capping, 3' end polyadenylation, and folding within an RNA protein complex (ribonucleoprotein particle or RNP).

Another mechanism of protection is ribonuclease inhibitor (RI), which comprises a relatively large fraction of cellular protein (~0.1%) in some cell types, and which binds to certain ribonucleases with the highest affinity of any protein-protein interaction; the dissociation constant for the RI-RNase A complex is ~20 fM under physiological conditions. RI is used in most laboratories that study RNA to protect their samples against degradation from environmental RNases.

Similar to restriction enzymes, which cleave highly specific sequences of double-stranded DNA, a variety of endoribonucleases that recognize and cleave specific sequences of single-stranded RNA have been recently classified.

RNases play a critical role in many biological processes, including angiogenesis and self-incompatibility in flowering plants (angiosperms). Also, RNases in prokaryotic toxin-antitoxin systems are proposed to function as plasmid stability loci, and as stress-response elements when present on the chromosome.

StephenW

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是大家没眼光还是大家都太傻谁知道?

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StephenW 发表于 2015-4-28 21:43
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Publications and presentations resulting from this thesis
Manuscripts in preparation:

这里列的文献我实在无力吐槽。2009年法国Tour那次会议恰恰就是我第一次接触他们的时候，大概也恰恰是他们第一次专业会议登场却被广泛质疑的开端。如果仔细看他们的结果就会发现动物实验设计和数据混乱不堪，因为化合物的毒性问题还死了不少北京鸭。我当时提出用更标准化更被业界所接受的一系列模型来验证他们的结果，所得到的答复，大家都已经知道了。

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StephenW 发表于 2015-4-28 22:50
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核糖核酸酶（英语：ribonuclease，常用縮寫：RNase）或稱RNA酶，是一種可將RNA水解 ...

我觉得你好像还是没搞清一个基本的概念，RNA病毒不是在那里等着被降解，而是在不断在自我复制，不断产生新的病毒RNA。病毒RNA当然会被降解，病毒也有自己的办法保护RNA不被降解的那么快，最终只要单位时间内复制新产生的RNA多于或等于被降解的RNA，那么感染就是一直持续。在病毒复制没有被抑制的情况下，就是这么个情况。

这不是很清楚明白的事吗，怎么搞得理解起来这么复杂？

StephenW

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这里列的文献我实在无力吐槽 你的决定
Prof Allison Jilbert是著名鸭肝炎研究.

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StephenW 发表于 2015-4-28 22:52
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是大家没眼光还是大家都太傻谁知道?

我可能傻，我可能没眼光，但我真心不相信这么些个大公司大专家，都没眼光都这么傻。如果真的是这样，我觉得你也不用期待有任何治愈HBV的可能了。

StephenW

HBVCURER 发表于 2015-4-28 23:06
我觉得你好像还是没搞清一个基本的概念，RNA病毒不是在那里等着被降解，而是在不断在自我复制，不断产生 ...

我觉得你好像还是没搞清一个基本的概念??

HDV始终在细胞内, 杀死细胞?

StephenW

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都没眼光都这么傻

不是所有都没眼光 - 他们有两个机构资金的支持，看看他们新的科学顾问的质量(e.g.  Dr Trepo)，当然他们可以都傻.

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StephenW 发表于 2015-4-28 23:07
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这里列的文献我实在无力吐槽 你的决定

著名研究员就不能被吐槽？说实话当时吐槽他最狠的的是比他更著名的多位研究员，我还清楚记得场面极为滑稽，很多人都抢着提问，都在质疑某些实验设计和结果，最后时间用完了又拖了很久，实在没办法，主席说你们还是下面去讨论吧。BTW，在HBV领域，Prof. Allison Jilbert只怕远谈不上著名，好像至今也只是Associate Professor。当然，我无意用title来判断一个人的学术水平，只是举个例子。