最新研究发现hbsag定量和cccDNA无关

alex1234

别愁 发表于 2011-6-29 09:50
“结论提示hbsag的复制和DNA, cccDNA是独立的。”

如果是这样，那HBsAg定量越低，停药反弹可能性越小（或 ...

我看了感觉就是定量没用，不抗病毒的小三阳测alt，抗病毒的测血清DNA。

放牛哥哥

alex1234 发表于 2011-6-29 08:11
在未接受过抗病毒的人中，血清hbsag和肝细胞中hbsag浓度正相关，和血清DNA弱相关，和cccDNA，肝细胞hbv不相 ...

能否给个链接或贴出全篇文章，谢谢！

负负得正

alex1234 发表于 2011-6-29 08:56
研究还发现，未治疗的小阳cccDNA和ALT正相关，看来肝功能更有意义。

是正相关吗？ALT越高，cccDNA也越高？

alex1234

摘要http://www.jhep-elsevier.com/article/S0168-8278(10)01088-3/abstract

alex1234

HEPATOLOGY是肝病研究领域最权威的杂志了。

放牛哥哥

alex1234 发表于 2011-6-29 11:05
HEPATOLOGY是肝病研究领域最权威的杂志了。

我想下载pdf全文，看个究竟，但网站提示，要我花31$进行购买！他娘的，一个文章要卖200人民币哦。
摘要是免费的：

放牛哥哥

alex1234 发表于 2011-6-29 10:23
摘要http://www.jhep-elsevier.com/article/S0168-8278(10)01088-3/abstract

http://www.jhep-elsevier.com/article/S0168-8278(10)01088-3/pdf
Background & Aims

Translation of HBsAg depends on transcription of the appropriate mRNAs from cccDNA, but its relation to other hepatitis B virus (HBV) replication parameters is not known, inasmuch as integrated sequences of HBV-DNA may also contribute to its serum levels, especially in HBeAg-negative chronic hepatitis B (CHB) patients.

Methods

We investigated HBsAg serum levels, its hepatocellular expression, and their relationship to HBV replicative- and host-response parameters before treatment in 54 HBeAg-negative CHB patients and in 15 of them after 40.1±33.3months of virological response on oral antiviral (NUC) therapy also. Liver cccDNA and HBV-DNA quantitation, HBsAg- and HBcAg-immunostaining were performed in the same needle biopsy material, while serum HBsAg and HBV-DNA levels were measured in samples drawn on the day of liver biopsy.

Results

In untreated patients, serum HBsAg correlated positively with HBsAg-positive hepatocytes/mm2 (p=0.003) and weakly with serum HBV-DNA, but not with cccDNA, liver HBV-DNA, HBcAg-positive hepatocytes/mm2, or ALT. cccDNA correlated significantly with liver HBV-DNA (p<0.00001), ALT (p=0.001), and serum HBV-DNA levels (p=0.012) but not with liver HBsAg or HBcAg. Antiviral therapy decreased serum HBsAg levels by 79.6% (p=0.012) and liver HBV-DNA by 84.4% (p=0.026) in paired comparisons and, as expected, significantly decreased serum HBV-DNA and ALT levels, but not cccDNA.

Conclusions

In untreated HBeAg-negative CHB, serum HBsAg levels reflect liver HBsAg, but not cccDNA or liver HBV-DNA, suggesting that they are not solely dependent on the replicative cycle of HBV. Effective NUC therapy for 3.34years significantly lowers serum HBsAg and liver HBV-DNA, but not cccDNA.

Keywords: Serum HBsAg levels, HBeAg-negative chronic hepatitis B, cccDNA, Total liver HBV DNA, Serum HBV DNA, HBsAg-immunostaining, HBcAg-immunostaining

我随心动

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还是在这个版面讨论更加合适。谢谢！

StephenW

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lifevendor 已翻译了文章的摘要.

lifevendor

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注意这里面治疗对象是HBeAg阴性的慢性HBV患者。