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发表于 2008-4-1 11:02
谢谢liver GZ。
http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt1396.html
这个是摘要(abstract)，
全文可能过一阵才能看到 （当然可以购买，不过过一阵应可看到）
http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt1396.html
http://www.nature.com/search/exe ... phrase&sp-p=all
这个是摘要：
Article abstract
Nature Biotechnology
Published online: 30 March 2008 | doi:10.1038/nbt1396
Resolution of liver cirrhosis using vitamin A–coupled liposomes to deliver siRNA against a collagen-specific chaperone
Yasushi Sato1,2, Kazuyuki Murase1,2, Junji Kato1,2, Masayoshi Kobune1, Tsutomu Sato1, Yutaka Kawano1, Rishu Takimoto1, Kouichi Takada1, Koji Miyanishi1, Takuya Matsunaga1, Tetsuji Takayama1 & Yoshiro Niitsu1
Abstract
There are currently no approved antifibrotic therapies for liver cirrhosis. We used vitamin A–coupled liposomes to deliver small interfering RNA (siRNA) against gp46, the rat homolog of human heat shock protein 47, to hepatic stellate cells. Our approach exploits the key roles of these cells in both fibrogenesis as well as uptake and storage of vitamin A. Five treatments with the siRNA-bearing vitamin A–coupled liposomes almost completely resolved liver fibrosis and prolonged survival in rats with otherwise lethal dimethylnitrosamine-induced liver cirrhosis in a dose- and duration-dependent manner. Rescue was not related to off-target effects or associated with recruitment of innate immunity. Receptor-specific siRNA delivery was similarly effective in suppressing collagen secretion and treating fibrosis induced by CCl4 or bile duct ligation. The efficacy of the approach using both acute and chronic models of liver fibrosis suggests its therapeutic potential for reversing human liver cirrhosis.
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1. Fourth Department of Internal Medicine, Sapporo Medical University, School of Medicine, Sapporo, 060-8543, Japan.
2. These authors contributed equally to this work.
Correspondence to: Yoshiro Niitsu1 e-mail: [email protected]
我试图和作者联系看看是否能email我一个digital 拷贝作为非商业用途。
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这个是路透社英国香港发表的新闻。 主要关键在介绍星状细胞因为同时可以吸收储存维生素A，人们利用这个让其“打开”门户使得阻碍剂进入关闭胶原蛋白制作...。
Man-made molecules reverse liver cirrhosis in rats
Sun Mar 30, 2008 6:02pm BST
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By Tan Ee Lyn
HONG KONG (Reuters) - Scientists in Japan have designed artificial molecules that when used with rats successfully reversed liver cirrhosis, a serious chronic disease in humans that until now can only be cured by transplants.
Cirrhosis is the hardening or scarring of the liver, and is caused by factors such as heavy drinking and Hepatitis B and C. The disease is especially serious in parts of Asia, including China.
Cirrhosis occurs when a class of liver cells starts producing collagen, a fibrous material that toughens skin and tendons. Such damage cannot be reversed although steps can be taken to prevent further damage. In advanced cases, transplants are the only way out.
In the journal Nature Biotechnology, the researchers said they designed molecules that can block collagen production by liver "stellate cells," which are also known to absorb vitamin
A.
The scientists then loaded the molecules into carriers that were coated with vitamin A, which tricked the stellate cells into absorbing the molecules.
"By packaging the (molecules) in carriers coated with vitamin A, they tricked the stellate cells into letting in the inhibitor, which shut down collagen secretion," the researchers wrote.
In the study, the researchers induced liver cirrhosis in rats and then injected them with the vitamin A-laced molecules.
"We were able to completely eradicate the fibrosis by injecting this agent ... we cured them of the cirrhosis," Yoshiro Niitsu at the Sapporo Medical University School of Medicine in Japan said in a telephone interview.
"The liver is such an important organ, after you remove the fibrosis, the liver by itself starts to regenerate tissues. So liver damage is reversible."
Explaining how the damage reversal came about, Niitsu said: "Liver is itself responsible for the production and deposition of collagen, it also secretes certain enzymes that dissolve collagen ... dissolve the fibrosis which has already been deposited in the tissues."
Niitsu was hopeful that the molecules would provide a cure for cirrhosis patients in time.
"We hope it (a drug) will be ready for humans in a few years," he said.
(Reporting by Tan Ee Lyn; Editing by Alistair Scrutton)
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