标题: Molecular epidemiological study of hepatitis B virus among [打印本页] 作者: liver411 时间: 2010-6-28 05:13 标题: Molecular epidemiological study of hepatitis B virus among
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J Med Virol. 2010 Jun 22;82(8):1341-1349. [Epub ahead of print]
Molecular epidemiological study of hepatitis B virus among migrant workers
from Cambodia, Laos, and Myanmar to Thailand.
Sa-Nguanmoo P, Tangkijvanich P, Thawornsuk N, Vichaiwattana P,
Prianantathavorn K, Theamboonlers A, Tanaka Y, Poovorawan Y.
Center of Excellence in Clinical Virology, Faculty of Medicine, Department of
Pediatrics, Chulalongkorn University, Bangkok, Thailand.
Abstract
Although hepatitis B virus (HBV) infection is endemic in Southeast Asia,
molecular epidemiological data on HBV circulating in some countries are
limited. The aims of this study were to evaluate the seroprevalence of HBV and
its genetic variability among migrant workers from Cambodia, Laos, and Myanmar
in Thailand. Sera collected from 1,119 Cambodian, 787 Laotian, and 1,103
Myanmarese workers were tested for HBsAg. HBV DNA was amplified and the
pre-S/S region was sequenced for genotyping and genetic mutation analysis.
HBsAg was detected in 282 (9.4%). The prevalence of HBsAg among migrant
workers from Cambodia, Laos, and Myanmar was 10.8%, 6.9%, and 9.7%,
respectively. Of 224 subjects positive for HBV DNA, 86% were classified as
genotype C (99% were sub-genotype C1) and 11.6% were genotype B (30.8%, 34.6%,
and 30.8% were sub-genotypes B2, B3, and B4, respectively). Various point
mutations in the "a" determinant region were detected in approximately 18% of
these samples, of which Ile126Ser/Asn was the most frequent variant.
Sequencing analysis showed that 19.1% of samples had pre-S mutations, with
pre-S2 deletion as the most common mutant (7.7%) followed by pre-S2 start
codon mutation (3.8%) and both pre-S2 deletion and start codon mutation
(3.3%). High prevalence of HBV infection (approximately 7-11%) was found among
migrant workers from Cambodia, Laos, and Myanmar, which may reflect the
current seroprevalence in their respective countries. The data also
demonstrated that HBV sub-genotype C1 was the predominant strain and various
mutations of HBV occurring naturally were not uncommon among these populations.
J. Med. Virol. 82:1341-1349, 2010. (c) 2010 Wiley-Liss, Inc.
PMID: 20572086 [PubMed - as supplied by publisher]
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