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标题: 新疆农牧区青少年OBI病毒S基因分子进化特征 [打印本页]

作者: StephenW    时间: 2022-12-13 20:04     标题: 新疆农牧区青少年OBI病毒S基因分子进化特征

新疆农牧区青少年OBI病毒S基因分子进化特征
Xinwei Qi 1 , Jianghong Dai 2 , Xiaoran Wang 1 , Mei Wang 3 , Wang Ying 4
隶属关系
隶属关系

     1个
     新疆医科大学第一附属医院中亚高发疾病发病机制及防治国家重点实验室,新疆 乌鲁木齐 830011
     2个
     新疆医科大学公共卫生学院,新疆 乌鲁木齐 830011
     3个
     民航医院,新疆 乌鲁木齐 830011
     4个
     新疆医科大学第一附属医院中亚高发疾病发病机制及防治国家重点实验室,新疆 乌鲁木齐 830011 电子地址:[email protected]

     PMID:36503123 DOI:10.1016/j.meegid.2022.105395

抽象的

目的:了解新疆农牧区青少年乙型肝炎病毒(HBV)实际感染率、隐匿性HBV感染率(OBI)及OBI病毒S基因的分子进化特征。

方法:对居住在农牧区的青少年人群进行横断面问卷调查。 在位于乌鲁木齐县盘房沟乡、水西沟村和庙沟村的三所中心学校的九年义务制学生中,采集了符合条件的静脉血样(3-5 毫升)。 采用了群体抽样,并获得了参与学生的知情同意。 通过电化学发光对所有血清样品进行定性乙型肝炎表面抗原 (HBsAg) 检测。 随后,通过巢式聚合酶链反应(PCR)扩增HBV S基因,纯化阳性PCR产物; 然后扩增目标基因序列。 使用 MEGA 11 软件对目标基因序列进行分子进化表征。

结果:总的来说,有 1712 名受试者被纳入。 HBsAg携带率和OBI感染率分别为1.93%(33/1712)和6.13%(103/1679)。 HBsAg(-)样本包括103株OBI毒株,其中B基因型毒株占80.58%(83/103;ayw1血清型1例,adw2血清型82例),C基因型毒株占14.56%(15/103) ;adw2血清型1例,adrq+血清型14例),D基因型菌株占4.85%(5/103;adw2血清型1例,ayw2血清型4例)。 在以下基因的“a”决定区检测到突变:P127S、G130R 和 N146S(B 基因型 OBI 菌株); T126I 和 T143S(C 基因型 OBI 菌株); T126I、P127S、F134Y 和 T143S(D 基因型 OBI 菌株)。

结论:一定比例的年轻人感染了OBI毒株。 OBI菌株的B基因型是可能的显性基因型。 OBI菌株在“a”决定区有氨基酸突变,很可能会发生抗原性和免疫原性的变化。 必须更加注意防止因 OBI 引起的问题。

关键词:青少年人口; 分子进化; 隐匿性乙型肝炎病毒感染 (OBI); S基因。

版权所有 © 2022。由 Elsevier B.V. 出版。
利益冲突声明

竞争利益声明 本文的作者之间没有利益冲突。
作者: StephenW    时间: 2022-12-13 20:04

Molecular evolutionary characteristics of OBI virus S gene among the adolescent population in rural and pastoral areas of Xinjiang Province
Xinwei Qi  1 , Jianghong Dai  2 , Xiaoran Wang  1 , Mei Wang  3 , Ying Wang  4
Affiliations
Affiliations

    1
    State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang, China.
    2
    School of Public Health of Xinjiang Medical University, Urumqi 830011, Xinjiang, China.
    3
    Civil Aviation Hospital, Urumqi 830011, Xinjiang, China.
    4
    State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang, China. Electronic address: [email protected].

    PMID: 36503123 DOI: 10.1016/j.meegid.2022.105395

Abstract

Objective: To determine the actual hepatitis B virus (HBV) infection rate, occult HBV infection (OBI) rate, and molecular evolutionary characteristics of the OBI virus S gene in the adolescent population living in rural and pastoral areas of Xinjiang Province.

Methods: A cross-sectional questionnaire survey was conducted among the adolescent population living in the farming and herding areas. Venous blood samples (3-5 mL) were collected from eligible students in three central schools located in Panfanggou Township, Shuixigou Village, and Miaolgou Village, all in Urumqi County, in the nine-year compulsory system. Clustersampling in a population was adopted, and informed consent was obtained from the participating students. All serum samples were qualitatively tested for hepatitis B surface antigen (HBsAg) by electrochemiluminescence. Subsequently, the HBV S gene was amplified by nested polymerase chain reaction (PCR), and the positive PCR products were purified; the target gene sequences were then amplified. Molecular evolutionary characterization of the target gene sequences was performed using MEGA 11software.

Results: Overall, 1712 subjects were enrolled. The HBsAg carrier rate and OBI infection rate were 1.93% (33/1712) and 6.13% (103/1679), respectively. HBsAg (-) samples included 103 OBI strains, of which B-genotype strains accounted for 80.58% (83/103; 1 case of ayw1 serotype and 82 cases of adw2 serotype), C-genotype strains accounted for 14.56% (15/103; 1 case of adw2 serotype and 14 cases of adrq+serotype), and D-genotype strains accounted for 4.85% (5/103; 1 case of adw2 serotype and 4 cases of ayw2 serotype). Mutations were detected in the "a" determinant region of the following genes: P127S, G130R, and N146S (B-genotype OBI strains); T126I and T143S (C-genotype OBI strains); T126I, P127S, F134Y, and T143S (D-genotype OBI strains).

Conclusion: A certain proportion of young people are infected with OBI strains. The B-genotype of OBI strains is the possible dominant genotype. OBI strains have amino acid mutations in the "a" determinant region, and they are likely to undergo a change in their antigenicity and immunogenicity. More attention must be paid to prevent problems due to OBI.

Keywords: Adolescent population; Molecular evolution; Occult hepatitis B virus infection (OBI); S gene.

Copyright © 2022. Published by Elsevier B.V.
Conflict of interest statement

Declaration of Competing Interest There is no conflict of interest between the authors of this article.

作者: StephenW    时间: 2022-12-13 20:04

https://doi.org/10.1016/j.meegid.2022.105395




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