A single birth dose of Hepatitis B vaccine induces polyfunctional CD4+ T helper cells
Julia Strandmark 1 , Alansana Darboe 1 , Joann Diray-Arce 2 3 , Rym Ben-Othman 4 , Sofia M Vignolo 2 , Shun Rao 2 , Kinga K Smolen 2 3 , Geert Leroux-Roels 5 , Olubukola T Idoko 1 , Guzmán Sanchez-Schmitz 2 3 , Al Ozonoff 2 3 6 , Ofer Levy 2 3 6 , Tobias R Kollmann 4 , Arnaud Marchant 7 , Beate Kampmann 1 8
Affiliations
Affiliations
1
Vaccines & Immunity Theme, Medical Research Council (MRC) Unit The Gambia at London School of Hygiene & Tropical Medicine (LSHTM), Fajara, Gambia.
2
Precision Vaccines Program, Boston Children's Hospital, Boston, MA, United States.
3
Department of Pediatrics, Harvard Medical School, Boston, MA, United States.
4
Department of Paediatrics, University of British Columbia, Vancouver, BC, Canada.
5
Center for Vaccinology, Ghent University Hospital, Ghent, Belgium.
6
Klarman Cell Observatory & Global Health Initiative, Broad Institute of the Massachusetts Institute of Technology (MIT) & Harvard, Cambridge, MA, United States.
7
Institute for Medical Immunology, Université Libre de Bruxelles, Brussels, Belgium.
8
The Vaccine Centre, London School of Hygiene and Tropical Medicine, London, United Kingdom.
A single birth-dose of Hepatitis B vaccine (HepB) can protect newborns from acquiring Hepatitis B infection through vertical transmission, though several follow-up doses are required to induce long-lived protection. In addition to stimulating antibodies, a birth-dose of HepB might also induce polyfunctional CD4+ T-cells, which may contribute to initial protection. We investigated whether vaccination with HepB in the first week of life induced detectable antigen-specific CD4+ T-cells after only a single dose and following completion of the entire HepB vaccine schedule (3 doses). Using HBsAg- stimulated peripheral blood mononuclear cells from 344 infants, we detected increased populations of antigen-specific polyfunctional CD154+IL-2+TNFα+ CD4+ T-cells following a single birth-dose of HepB in a proportion of infants. Frequencies of polyfunctional T-cells increased following the completion of the HepB schedule but increases in the proportion of responders as compared to following only one dose was marginal. Polyfunctional T-cells correlated positively with serum antibody titres following the birth dose (day30) and completion of the 3-dose primary HepB vaccine series (day 128). These data indicate that a single birth dose of HepB provides immune priming for both antigen-specific B- and T cells.
Keywords: BCG; CD154; CD4 + T helper cell; Hepatitis B vaccine; T-cell activation; antibody titres; neonate.
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. 作者: StephenW 时间: 2022-11-26 13:41