慢性乙型肝炎患者细胞因子水平和T细胞表面分子的变化及其

StephenW

Changes of cytokine levels and T cell surface molecules in patients with chronic hepatitis B and the association with functional cure
Zhen Xu  1   2   3 , Ji-Zong Lin  4 , Ying-Fu Zeng  1   2   3 , Xiao-Hua Yang  1   2   3 , Zhe-Bin Wu  1   2   3 , Zhao-Xia Hu  1   2   3 , Qi-Yi Zhao  1   2   3 , Jing Liu  1   2   3 , Zhi-Liang Gao  1   2   3
Affiliations
Affiliations

    1
    Department of Infectious Diseases, the Third Affiliated Hospital of Sun Yat-sen, University, Guangzhou, Guangdong, 510630, China.
    2
    Guangdong Key Laboratory of Liver Disease Research, the Third Affiliated, Hospital of Sun Yat-sen University, Guangzhou, 510630, China.
    3
    Key Laboratory of Tropical Disease Control (Sun Yat-Sen University), Ministry of Education, Guangzhou, Guangdong, 510080, China.
    4
    General Surgery Department, Lingnan Hospital the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, 510630, China.

    PMID: 33913556 DOI: 10.1002/jmv.27041

Abstract

Background: This study aimed to examine changes in levels of cytokine and T cell surface molecules in chronic hepatitis B (CHB) patients receiving sequential interferon therapy following 1-year nucleos(t)ide analogues (NAs) treatment.

Methods: Cytokine levels were measured in 30 patients, and T cell surface molecule expression was measured in 48 patients receiving sequential interferon therapy and 24 patients only receiving NA mono-therapy. A HBsAg titer of < 0.05 IU/ml was defined as a "functional cure".

Results: In the cured group (HBsAg < 0.05 IU/ml), a decreasing probability was observed in IFN-γ (after week 0), and IL-22 and IP-10 (after week 12). In the non-cured group (HBsAg ≥ 0.05 IU/ml), a probability of slightly decreasing was observed for IFN-γ (after week 12), and a probability of increasing IP-10 concentration (after week 0) was observed. GEE analyses showed significant differences in the levels of IL-10, IL-23, CCL-3, IL-1β, IL-2, and IL-12P70 between the two groups. In GEE analysis, there were significant differences in expressions of CD45RO+ between the cured group and the non-cured group. The frequencies of T cells expressing Tim-3, CD62L, and CD152 were significantly lower in the sequential interferon therapy group than in the NA mono-therapy group.

Conclusions: Changes in cytokine levels (IFN-γ, IP-10, IL-10, IL-23, CCL-3, IL-1β, IL-2, and IL-12P70) and T cell surface molecules (CD45RO+) may predict HBsAg seroconversion in CHB patients receiving sequential interferon therapy. The period from week 12 to week 24 during sequential interferon therapy may be a critical time of immune status change. This article is protected by copyright. All rights reserved.

Keywords: Chronic hepatitis B; HBsAg seroconversion; co-inhibitory molecule; cure; cytokines; nucleos(t)ide analogues; sequential interferon therapy.

This article is protected by copyright. All rights reserved.

StephenW

慢性乙型肝炎患者细胞因子水平和T细胞表面分子的变化及其与功能性治愈的关系
许振1 2 3，林继宗4，曾颖甫1 2 3，杨小华1 2 3，吴哲彬1 2 3，胡兆霞1 2 3，赵启义1 2 3，刘静1 2 3，高志良1 2 3
隶属关系
隶属关系

    1个
    大学中山大学附属第三医院感染科，广东广州510630
    2个
    中山大学附属第三医院广东省肝病研究重点实验室，广东广州510630
    3
    中山大学热带病防治重点实验室（中山大学），广东广州510080
    4
    中山大学附属第三医院岭南医院普外科，广东广州510630

    PMID：33913556 DOI：10.1002 / jmv.27041

抽象的

背景：本研究旨在检查接受1年核苷酸（t）类似物（NAs）治疗后接受序贯干扰素治疗的慢性乙型肝炎（CHB）患者中细胞因子和T细胞表面分子水平的变化。

方法：在30例患者中测量细胞因子水平，在48例接受序贯干扰素治疗的患者和24例仅接受NA单一疗法的患者中测量T细胞表面分子表达。 ＜0.05 IU / ml的HBsAg滴度定义为“功能性治愈”。

结果：在治愈组（HBsAg <0.05 IU / ml）中，观察到IFN-γ（第0周后），IL-22和IP-10（第12周后）降低的可能性。在非治愈组（HBsAg≥0.05 IU / ml）中，观察到IFN-γ略有降低的可能性（在第12周后），而IP-10浓度升高的可能性（在第0周后）。 GEE分析显示两组之间IL-10，IL-23，CCL-3，IL-1β，IL-2和IL-12P70的水平存在显着差异。在GEE分析中，治愈组和未治愈组之间CD45RO +的表达存在显着差异。序贯干扰素治疗组中表达Tim-3，CD62L和CD152的T细胞的频率显着低于NA单药治疗组。

结论：细胞因子水平（IFN-γ，IP-10，IL-10，IL-23，CCL-3，IL-1β，IL-2和IL-12P70）和T细胞表面分子（CD45RO +）的变化可能预示着接受序贯干扰素治疗的CHB患者的HBsAg血清转化。连续干扰素治疗期间从第12周到第24周的时间可能是免疫状态改变的关键时期。本文受版权保护。版权所有。

关键字：慢性乙型肝炎； HBsAg血清转化；共抑制分子治愈;细胞因子核苷（核苷酸）类似物;序贯干扰素治疗。

本文受版权保护。版权所有。