An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
Mohammad Haque 1 , Xiaofang Xiong 1 , Fengyang Lei 2 , Jugal Kishore Das 1 , Jianxun Song 1
Affiliations
Affiliations
1
Department of Microbial Pathogenesis and Immunology, Texas A&M University Health Science Center, MREB II, Room 3344, 8447 Riverside Pkwy, Bryan, TX 77807, USA.
2
Department of Ophthalmology, Harvard Medical School, Boston, MA 02215, USA.
In T cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8+ cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag+ cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque et al. (2020).