基于脱细胞人肝支架的三维培养系统促进HBV感染

StephenW

J Biomed Mater Res A. 2019 Apr 8. doi: 10.1002/jbm.a.36690. [Epub ahead of print]
Decellularized Human Liver Scaffold-Based Three-dimensional Culture System Facilitate HBV Infection.
Zhang Z1,2, Xu H1,2, Mazza G3, Zhang M4, Frenguelli L3, Liu Q1,2, Al-Akkad W3, Ren J5,6, Zhao R1,2, Ren F5,6, Chen X1,7, Huang A5,6, Chen J5,6.
Author information

1
    Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, ChongQing, China.
2
    Department of Infectious Disease, Children's Hospital of ChongQing Medical University, ChongQing, China.
3
    UCL Institute for Liver and Digestive Health, Royal Free Hospital., University College London, London, UK.
4
    Department of Hepatobiliary Surgery, Children's Hospital of ChongQing Medical University, ChongQing, China.
5
    The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, ChongQing, China.
6
    Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, ChongQing, 400016, China.
7
    The General Gard, Children's Hospital of ChongQing Medical University, ChongQing, China.

Abstract

Hepatitis B virus (HBV) study is hampered by lacking of idea cell model which support effective HBV infection, and meanwhile recapitulate hepatocyte biology function in vivo. In this study, we developed decellularized human liver scaffolds for cell culture and further applied for HBV infection. As a result, primary human hepatocytes (PHH) engrafted into liver scaffolds and maintained differentiation with stable albumin secretion and liver specific gene expression. Comparing to mono-layer cell culture, scaffold-based 3D culture system significantly augment HBV DNA (including cccDNA), RNA level as well as HBsAg secretion. Moreover, HepG2-NTCP cells cultured on 3D system exhibited higher infection efficiency and longer infection period in vitro. In addition, HBV DNA level was suppressed when anti-HBV medicine Entecavir (ETV) introduced into HepG2-NTCP 3D system. Herein, we evaluted the potential of decellularized human liver scaffold-based in 3D cell culture and disclosed that scaffold-based 3D culture system can facilitate HBV infection in vitro. This 3D culture system could be further applied in HBV related study. This article is protected by copyright. All rights reserved.

This article is protected by copyright. All rights reserved.
KEYWORDS:

3D culture; decellularized human liver scaffold; hepatitis B virus

PMID:
    30963688
DOI:
    10.1002/jbm.a.36690

StephenW

J Biomed Mater Res A. 2019 Apr 8. doi：10.1002 / jbm.a.36690。 [印刷前的电子版]
基于脱细胞人肝支架的三维培养系统促进HBV感染。
Zhang Z1,2，Xu H1,2，Mazza G3，Zhang M4，Frenguelli L3，Liu Q1,2，Al-Akkad W3，Ren J5,6，Zhao R1,2，Ren F5,6，Chen X1,7，Huang A5,6，陈J5,6。
作者信息

1
教育部儿童发育与疾病重点实验室，重庆市儿科重点实验室，重庆市儿童发育与疾病国际科技合作中心，重庆，中国。
2
重庆医科大学附属儿童医院传染病科，重庆，中国。
3
英国伦敦大学学院皇家自由医院UCL肝脏和消化系统健康研究所。
4
重庆医科大学附属儿童医院肝胆外科，重庆，中国。
五
中国重庆市教育部指定的传染病分子生物学重点实验室。
6
重庆医科大学附属第二医院感染科，病毒性肝炎研究所，重庆400016
7
重庆医科大学附属儿童医院，重庆，中国。

抽象

缺乏支持有效HBV感染的想法细胞模型阻碍乙型肝炎病毒（HBV）研究，并且血液在体内重现肝细胞生物学功能。在这项研究中，我们开发了细胞培养的脱细胞人肝支架，并进一步应用于HBV感染。 </ br> </ br>此外，当抗HBV药物恩替卡韦（ETV）引入HepG2-NTCP 3D系统时，HBV DNA水平受到抑制。在此，我们评估了基于3D细胞培养的脱细胞人肝支架的潜力，并且公开了支架-ba Sed 3D培养系统可以促进体外HBV感染。该3D培养系统可进一步应用于HBV相关研究。本文受版权保护。

本文受版权保护。版权所有。
关键词：

3D文化;脱细胞人肝支架;乙型肝炎病毒

结论：
30963688
DOI：
10.1002 / jbm.a.36690