乙型肝炎病毒感染患者树突状细胞功能受损的特点。

StephenW

Hepatology. 2019 Apr 2. doi: 10.1002/hep.30637. [Epub ahead of print]
Characteristics of impaired dendritic cell function in patients with hepatitis B virus infection.
Yonejima A1, Mizukoshi E1, Tamai T1, Nakagawa H1, Kitahara M1, Yamashita T1, Arai K1, Terashima T1, Iida N1, Fushimi K1, Okada H1, Yamashita T1, Sakai Y1, Honda M1, Kaneko S1.
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Abstract

Dendritic cells are antigen-presenting cells with a central role in host immune response. This study analyzed gene expression and dendritic cell function in hepatitis B virus (HBV) patients, functions impaired due to HBV, and identified the genes related to these functions. Peripheral blood mononuclear cells from 64 HBV patients and 19 healthy controls were analyzed. Peripheral blood dendritic cells were stained with antibodies against HLA-DR/Lin-1/CD123/CD11c and separated into plasmacytoid dendritic cells and myeloid dendritic cells by fluorescence-activated cell sorting. Using IFN-γ enzyme-linked immunospot assay, we analyzed the antigen-specific response in HBV-infected patients. Regarding dendritic cell function, we analyzed antigen-presenting capacity, cell migration capacity, phagocytic capacity, and cytokine production capacity. Dendritic cell gene expression was analyzed by microarray to identify genes related to dendritic cell function. No difference was found in the number of dendritic cells in peripheral blood between healthy participants and HBV patients. In cell surface marker analysis, CD80, CD83, CD86, CD40, and CCR7 expression levels in plasmacytoid dendritic cells were related to the HBV-specific T cell response. Dendritic cells from HBV patients exhibited decreases in antigen-presenting capacity, migration capacity, and cytokine production capacity. In gene expression analysis, immune-related genes with greatly reduced expression levels in chronic hepatitis B patients were identified. Of these genes, IL6ST expression level positively correlated with the dendritic cell surface marker expression level. Adjustment of the IL6ST expression level in dendritic cells and treatment with oncostatin M resulted in recovery of dendritic cell function. CONCLUSION: IL6ST expression was identified as one cause of decline in dendritic cell function in HBV patients. Adjustment of IL6 family cytokine signaling may be useful for recovering reduced dendritic cell function in HBV infection. This article is protected by copyright. All rights reserved.

This article is protected by copyright. All rights reserved.
KEYWORDS:

T cell; chronic hepatitis; gp130; immunotherapy; interleukin-6

PMID:
    30938456
DOI:
    10.1002/hep.30637

StephenW

肝病。 2019年4月2日2. doi：10.1002 / hep.30637。 [印刷前的电子版]
乙型肝炎病毒感染患者树突状细胞功能受损的特点。
Yonejima A1，Mizukoshi E1，Tamai T1，Nakagawa H1，Kitahara M1，Yamashita T1，Arai K1，Terashima T1，Iida N1，Fushimi K1，Okada H1，Yamashita T1，Sakai Y1，Honda M1，Kaneko S1。
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抽象

树突细胞是抗原呈递细胞，在宿主免疫应答中起重要作用。该研究分析了乙型肝炎病毒（HBV）患者的基因表达和树突细胞功能，HBV引起的功能受损，并鉴定了与这些功能相关的基因。分析来自64名HBV患者和19名健康对照的外周血单核细胞。外周血树突细胞用针对HLA-DR / Lin-1 / CD123 / CD11c的抗体染色，并通过荧光激活细胞分选分离成浆细胞样树突细胞和骨髓树突细胞。使用IFN-γ酶联免疫斑点测定，我们分析了HBV感染患者的抗原特异性反应。关于树突细胞功能，我们分析了抗原呈递能力，细胞迁移能力，吞噬能力和细胞因子产生能力。通过微阵列分析树突细胞基因表达以鉴定与树突细胞功能相关的基因。健康参与者和HBV患者之间的外周血中树突细胞的数量没有差异。在细胞表面标志物分析中，浆细胞样树突细胞中的CD80，CD83，CD86，CD40和CCR7表达水平与HBV特异性T细胞应答有关。来自HBV患者的树突细胞表现出抗原呈递能力，迁移能力和细胞因子产生能力的降低。在基因表达分析中，鉴定了在慢性乙型肝炎患者中表达水平大大降低的免疫相关基因。在这些基因中，IL6ST表达水平与树突细胞表面标志物表达水平正相关。调整树突细胞中的IL6ST表达水平和用制瘤素M处理导致树突细胞功能的恢复。结论：IL6ST表达被确定为HBV患者树突状细胞功能下降的一个原因。调节IL6家族细胞因子信号传导可用于恢复HBV感染中减少的树突细胞功能。本文受版权保护。版权所有。

本文受版权保护。版权所有。
关键词：

T细胞;慢性肝炎; gp130的;免疫治疗;白细胞介素-6

结论：
    30938456
DOI：
    10.1002 / hep.30637