January 2019, Volume 13, Issue 1, pp 75–83 | Cite as
Activity of IL-12/15/18 primed natural killer cells against hepatocellular carcinoma
Authors
Authors and affiliations
Lihui ZhuangRebecca J. FultonPauline RettmanA. Emre SayanJonathan CoadAymen Al-ShamkhaniSalim I. KhakooEmail author
Lihui Zhuang
1
Rebecca J. Fulton
1
Pauline Rettman
1
A. Emre Sayan
1
Jonathan Coad
1
Aymen Al-Shamkhani
1
Salim I. Khakoo
1Email authorView author's OrcID profile
1.Faculty of Medicine, Southampton General HospitalUniversity of SouthamptonSouthamptonUK
Open Access
Original Article
First Online: 22 November 2018
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Abstract
Background
Hepatocellular carcinoma (HCC) is common, but remains difficult to treat. Natural killer (NK) cells are cells of the innate immune system that have potent anti-cancer activity. Recent work has shown that stimulation with IL-12/15/18 leads to the generation of NK cells with enhanced functional and putative “memory” properties. We have investigated the activity of these NK cells against HCC cell lines in vitro and in a mouse model.
Methods
NK cells from healthy donors or individuals with HCC were activated with IL-12/15/18 in vitro and tested for cytotoxic activity against a panel of human HCC cell lines. IL-12/15/18 primed murine NK cells were then infused into a murine model of spontaneously arising HCC to test for anti-tumor activity.
Results
NK cells from patients and healthy controls had similar expression levels of activating and inhibitory NK cell receptors. However, proliferation of NK cells from HCC patients was weaker than healthy controls in response to IL-12/15/18 and IL-2 (p < 0.001 at day 9). In vitro, NK cells from both groups of individuals killed HCC targets to similar levels and this was unrelated to NKG2D expression. In a spontaneous model of HCC, IL-12/15/18 activated NK cells trafficked to the liver and resulted in lower levels of spontaneous HCC formation (p < 0.01).
Conclusion
Cytokine-primed NK cells from patients with HCC have similar levels of activity against HCC cell lines as those from healthy controls. This type of activated NK cell has immunotherapeutic potential against hepatocellular carcinoma.
Keywords
Innate immunity Immunotherapy Cytokines Liver Cancer 作者: StephenW 时间: 2019-2-19 19:36