Gene. 2019 Jan 7. pii: S0378-1119(19)30002-2. doi: 10.1016/j.gene.2018.12.054. [Epub ahead of print]
Hepatitis B virus X reduces hepatocyte apoptosis and promotes cell cycle progression through the Akt/mTOR pathway in vivo.
Wang X1, Huo B2, Liu J2, Huang X1, Zhang S2, Feng T3.
Author information
1
Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China; Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
2
Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China; School of Pharmaceutical Science, Chongqing Medical University, Chongqing 400016, China.
3
Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China; School of Pharmaceutical Science, Chongqing Medical University, Chongqing 400016, China. Electronic address: [email protected].
Abstract
Hepatitis B virus X (HBx), a viral onco-protein encoded by HBV, can promote oncogenesis of HCC. However, the mechanism of HBx in hepatocarcinogenesis is still unclear. In this study, we establish a new mouse model with normal immune system to investigate the role of HBx and its functional mechanisms under normal immune function. The animal model was established by injecting HBx-EGFP-14-19 cells into the hepatic portal vein of KM mice. To verify the mouse model, the expression of HBx in the liver tissue of mice was detected by qRT-PCR, western blotting and immunohistochemistry. The apoptosis index was calculated using the terminal deoxynucleotidyl transferase-dUTP nick-end labeling (TUNEL) assay, and the expression levels of apoptosis-related and cell cycle-related factors were measured. Moreover, expression of proteins in the protein kinase B/mammalian target of rapamycin (Akt/mTOR) signaling pathway was detected in HBx-EGFP-14-19 mice with and without use of an Akt inhibitor. The results showed the HBx was successfully overexpressed in liver of KM mice. After overexpressing HBx, the apoptosis index was downregulated in HBx-EGFP-14-19 liver tissue, and the expression levels of caspase-9 and Bad were reduced, but Bcl-xl was increased in HBx-EGFP-14-19 liver tissue. Overexpression of HBx increased the expression of the cyclin-dependent kinase 2 (CDK2), cyclinD1 and cyclinE. Moreover, compared with the low-level HBx group, p-Akt and p-mTOR were increased in the livers of mice with high levels of HBx. However, inactivation of apoptosis by overexpression of HBx was abolished by the treatment with an Akt inhibitor. These results indicate that HBx can induce anti-apoptosis mechanisms in hepatocytes in vivo, which is mediated by the Akt/mTOR signaling pathway.