Angew Chem Int Ed Engl. 2018 Aug 8. doi: 10.1002/anie.201807212. [Epub ahead of print]
Bioinspired Artificial Nanodecoys for Hepatitis B Virus.
Liu X1, Yuan L1, Zhang L1, Mu Y1, Li X1, Liu C1, Lv P1, Zhang Y1, Cheng T1, Yuan Q1, Xia N1, Chen X2, Liu G3.
Author information
1
CHINA.
2
UNITED STATES.
3
Center for Molecular Imaging and Translational Medicine, Xiang'An South Rd., Xiang'An District, CHINA.
Abstract
We report a facile route for fabricating a new class of nanomimics that overexpress hepatitis B virus (HBV) receptor via a natural biosynthetic procedure against HBV infection. We engineered a nine-transmembrane HBV-specific receptor, human sodium taurocholate co-transporting polypeptide (hNTCP), to naturally immobilize it onto the cellular surface and subsequently trigger the budding of hNTCP-anchoring membrane vesicles (hNTCP-MVs) that favor the HBV virion. We confirmed that hNTCP-MVs could rapidly block HBV infection in cell models. Furthermore, hNTCP-MVs treatment could effectively prevent viral infection, spreading, and replication in a human-liver-chimeric mouse model of HBV infection. Our findings demonstrate the receptor-mediated antiviral effect of hNTCP-MVs to trick HBV and offer novel opportunities for further development of antiviral strategies in nanomedicine.
KEYWORDS:
hepatitis B virus; infection inhibition; nanomedicine; nanovesicles; receptor