标题: 病毒粒子相关乙型肝炎病毒基因组中分歧的前序列和来自HBV e [打印本页] 作者: StephenW 时间: 2018-3-12 12:46 标题: 病毒粒子相关乙型肝炎病毒基因组中分歧的前序列和来自HBV e
Divergent preS sequences in virion associated hepatitis B virus genomes and subviral HBV surface antigen particles from HBV e antigen negative patients
Kai-Henrik Peiffer Lisa Kuhnhenn Bingfu Jiang Antonia Mondorf Johannes Vermehren Viola Knop Simone Susser Dirk Walter Julia Dietz Gert Carra ... Show more
The Journal of Infectious Diseases, jiy119, https://doi.org/10.1093/infdis/jiy119
Published:
08 March 2018
Abstract
Background
The hepatitis B virus (HBV) surface proteins (HBsAg) coat the viral particle and form subviral particles (SVPs). Loss of HBsAg represents a functional cure and is an important treatment goal.
Methods
We analyzed the impact of the HBV genotypes A-E and pre-S mutations on SVP expression in HBeAg negative chronic HBV infected patients. A HBV genome harboring a preS1-deletion was analyzed in hepatoma cells.
Results
We observed a genotype-specific ratio of the three surface proteins (SHBs/MHBs/LHBs), which reflects differences in the morphology and composition of SVPs. Deletions/mutations in the preS1/preS2 domain, detected in released viral genomes, did not affect the molecular weight of MHBs and LHBs in these patients. In contrast, LHBs molecular weight was altered in vitro using a HBV genome harboring a preS1-deletion derived from one of these patients.
Conclusion
Differences in composition of SVPs may result in a genotype-specific immunogenicity and pathogenesis. In the analyzed patients with preS-mutations secreted HBsAg and released viral genomes cannot be derived from the same genetic source. As viral genomes are derived from cccDNA, HBsAg is presumably derived from the integrated DNA. This important HBsAg source has to be considered for novel antiviral strategies in HBeAg negative chronic HBV infected patients. 作者: StephenW 时间: 2018-3-12 12:46
病毒粒子相关乙型肝炎病毒基因组中分歧的前序列和来自HBV e抗原阴性患者的亚病毒表面抗原颗粒
Kai-Henrik Peiffer Lisa Kuhnhenn Bingfu Jiang Antonia Mondorf Johannes Vermehren Viola Knop Simone Susser Dirk Walter Julia Dietz Gert Carra ...显示更多
“传染病杂志”，jiy119，https://doi.org/10.1093/infdis/jiy119
发布时间：
2018年3月08日