J Nanosci Nanotechnol. 2018 Feb 1;18(2):1083-1088. doi: 10.1166/jnn.2018.13955.
Immunoreaction-Mediated Aggregation of Gold Nanoparticles for Sensitive and Selective Assay of Hepatitis B Surface Antigen.Le DV1, Le VT1, Nguyen VC1, Jiang JH2, Dang KT3. Author information 1Faculty of Chemical Engineering, Industrial University of Ho Chi Minh City, Go Vap, Ho Chi Minh City 70000, Vietnam.2State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, P. R. China.3Institute of Research and Applied Technological Science, Dong Nai Technology University, Dong Nai 810000, Vietnam.
AbstractA rapid, sensitive and quantitative assay method for Hepatitis B surface antigen (HBsAg) is of paramount importance for the drug development and in the diagnosis of this disease. Here, we proposed a novel biosensor that sensitively and selectively screen Hepatitis B surface antigen. This strategy relies on the cross-linking aggregation of gold nanoparticles (AuNPs) that were decorated with Hepatitis B surface antibody (HBsAb) and Raman reporter 5-thio-nitrobenzoic acid (TNB) by Hepatitis B surface antigen. The immune reaction between HBsAb and HBsAg offers this strategy high specificity, and the use of AuNPs additionally allows a visual and homogeneous assay format, thus permitting improved simplicity and throughput of the assays. The selectivity and sensitivity in HBsAg assay were achieved with a wide linear response range from 0.5 ng/mL to 50 ng/mL and a detection limit of 0.2 ng/mL. The results indicated that this strategy can offer a simple, robust and convenient platform for HBsAg analysis and related biochemical studies with high sensitivity and selectivity.
一种快速，灵敏和定量的乙型肝炎表面抗原（HBsAg）检测方法对药物开发和诊断这种疾病至关重要。在这里，我们提出了一种新颖的生物传感器，可以灵敏地选择性筛选乙肝表面抗原。该策略依赖于由乙型肝炎表面抗原经乙型肝炎表面抗体（HBsAb）和拉曼记者5-硫代硝基苯甲酸（TNB）修饰的金纳米颗粒（AuNPs）的交联聚集。 HBsAb和HBsAg之间的免疫反应提供了这种策略的高度特异性，并且AuNPs的使用额外允许视觉和均质测定形式，从而允许测定的改进的简单性和通量。 HBsAg检测中的选择性和灵敏度可通过0.5 ng / mL到50 ng / mL的广泛线性响应范围和0.2 ng / mL的检测限实现。结果表明，该策略可以为HBsAg分析和相关生物化学研究提供一个简单，稳健和方便的平台，具有高灵敏度和高选择性。