Differentially expressed intrahepatic genes contribute to control of hepatitis B virus replication in the inactive carrier phase
Hongyan Liu Fahong Li Xiaoyong Zhang Jie Yu Jinyu Wang Jia Jia Xueping Yu Zhongliang Shen Zhenghong Yuan Xiaonan Zhang ... Show more
The Journal of Infectious Diseases, jix683, https://doi.org/10.1093/infdis/jix683
Published:
02 January 2018
Abstract
Background
The natural history of chronic HBV infection was divided into four phases. Patients in the inactive carrier status (IC) and immune tolerant (IT) phase had normal ALT levels but huge different viral loads. The mechanism underlying low viral replication status in IC phase is unknown.
Methods
We determined the intrahepatic transcriptomes of 83 chronic hepatitis B patients by microarray analysis of liver biopsies, and screened the effect of differentially regulated genes on HBV replication using specific siRNAs in vitro.
Results
The gene profile distinguishing active chronic hepatitis from IT and IC was predominantly composed of immune-related genes. The liver transcriptomes between the IT and IC phase were largely similar, and 109 expressed genes were significantly different. By performing systematic screening, 5 candidate genes including EVA1A, which were expressed at relative higher level in IC phase than IT, were identified to regulate HBV replication and gene expression in cellular models.
Conclusions
The immune-related pathways were up-regulated in the active chronic hepatitis phase but not in the IT and IC phase. A number of intrahepatic genes highly expressed in the IC phase may participate in the control of HBV replication and determine the inactive status of HBV infection. 作者: StephenW 时间: 2018-1-3 22:42
差异表达的肝内基因有助于在无活性的载体阶段控制乙型肝炎病毒的复制
Hongyan Liu Fahong李晓勇张杰于金玉王佳贾雪萍于忠良沉正宏袁晓楠张...显示更多
“传染病杂志”，jix683，https://doi.org/10.1093/infdis/jix683
发布时间：
2018年1月2日