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Front Cell Infect Microbiol. 2017 Mar 28;7:98. doi: 10.3389/fcimb.2017.00098. eCollection 2017.
Modulation of Tim-3 Expression by Antigen-Dependent and -Independent Factors on T Cells from Patients with Chronic Hepatitis B Virus Infection.
Dong J1, Yang XF2, Wang LX2, Wei X2, Wang AH3, Hao CQ2, Shen HJ2, Huang CX2, Zhang Y2, Lian JQ2.
Author information
1 Center for Infectious Diseases, Tangdu Hospital, Fourth Military Medical UniversityXi'an, China; Department of Ophthalmology and Otorhinolaryngology, Tenth Hospital of PLAWuwei, China.
2 Center for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University Xi'an, China.
3 Department of Epidemiology, School of Public Health, Fourth Military Medical University Xi'an, China.
Abstract
T-cell immunoglobulin domain and mucin domain-containing molecule-3 (Tim-3) was up-regulated on viral specific T cells and contributed to T cells exhaustion during chronic hepatitis B virus (HBV) infection. However, modulation of Tim-3 expression was still not fully elucidated. To evaluate the potential viral and inflammatory factors involved in the inductor of Tim-3 expression on T cells, 76 patients with chronic HBV infection (including 40 chronic hepatitis B [CHB] and 36 asymptomatic HBV carriers [AsC]) and 40 of normal controls (NCs) were enrolled in this study. Tim-3 expressions on CD4+ and CD8+ T cells were assessed in response to HBV-encoding antigens, HBV peptide pools, and common γ-chain (γc) cytokines stimulation by flow cytometry. HBV peptides and anti-CD3/CD28 directly induced Tim-3 expression on T cells. γc cytokines also drive Tim-3 up-regulations on both CD4+ and CD8+ T cells in patients with chronic HBV infection. However, γc cytokines did not enhance the Tim-3 inductions by either anti-CD3/CD28 or HBV peptides stimulation. Furthermore, γc cytokines-mediated Tim-3 induction could not be abrogated by γc cytokine receptor-neutralizing antibodies. The current results suggested that elevation of Tim-3 expression on T cells could be regulated by both antigen-dependent and -independent manner in patients with chronic HBV infection. The role of γc cytokines in modulation of inhibitory pathway might be evaluated as immunotherapies in humans.
KEYWORDS:
T cell receptor; T-cell immunoglobulin domain and mucin domain-containing molecule-3; common γ-chain cytokines; hepatitis B virus; immunomodulation
PMID:
28401068
PMCID:
PMC5368241
DOI:
10.3389/fcimb.2017.00098
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