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Serum hepatitis B core-related antigen as a good surrogate
marker of intrahepatic covalently closed circular
DNA during different phases of chronic hepatitis B virus
infection
En-Qiang Chen1,2, Shu Feng3, Ling-Bo Liang1,2, Ling-Yun Zhou1,2,
Ling-Yao Du1,2, Li-Bo Yan1,2, Meng-Lan Wang1,2, Chuan-Min Tao3,
Hong Tang1,2; 1Center of Infectious Diseases, West China Hospital
of Sichuan University, Chengdu, China; 2Division of Infectious
Diseases, State Key Laboratory of Biotherapy, Sichuan University,
Chengdu, China; 3Department of Laboratory Medicine, West
China Hospital, Sichuan University, Chengdu, China
Aim: This study aimed to investigate the correlation of serum
quantitative hepatitis B core-related antigen (qHBcrAg) with
intrahepatic cccDNA in the different phases of the natural
history of chronic hepatitis B (CHB), and thus reveal whether
serum qHBcrAg could be used as an surrogate marker of intrahepatic
cccDNA. Methods: A total of 139 CHB patients who
underwent liver biopsy were enrolled, consisting of 59 patients
in immune tolerance (IT) phase, 52 patients in immune clearance
(IC) phase, 20 patients in low-replication (LR) phase, and
8 patients in reactivation phase. All patients in IC phase have
received entecavir (ETV) therapy, and 32 of them undergone
a second liver biopsy at 24 months. In this study, the serum
qHBcrAg, qHBsAg, HBV DNA and intrahepatic cccDNA levels
were all quantitatively measured. Results: Among the 139
patients, serum qHBcrAg was strongly positive correlated with
intrahepatic cccDNA (r=0.929, P<0.001), which is superior
to that of serum qHBsAg (r=0.742, P<0.001) and HBV DNA
(r=0.854, P<0.001) with intrahepatic cccDNA. And similar
interesting findings were also observed in patients in IT, IC, LR
and reactivation phases. Among the 32 ETV-treated patients in
IC phase who undergone a second liver biopsy, the decline of
intrahepatic cccDNA was accompanied by changes in both
qHBcrAg and qHBsAg. However, as compared to qHBsAg,
the dynamic change of qHBcrAg was more strongly associated
with intrahepatic cccDNA-decline (r=0.665, P<0.001 for qHBcrAg;
and r=0.572, P=0.001 for qHBsAg). Conclusions: As
compared to either qHBsAg or HBV DNA, serum qHBcrAg has
a better positive correlation with intrahepatic cccDNA. And
serum qHBcrAg should be a good surrogate of intrahepatic
cccDNA of HBV.
The correlations of serum qHBcrAg (A), qHBsAg (B) and HBV
DNA (C) with intrahepatic cccDNA among different phases of
CHB.
Disclosures:
The following people have nothing to disclose: En-Qiang Chen, Shu Feng,
Ling-Bo Liang, Ling-Yun Zhou, Ling-Yao Du, Li-Bo Yan, Meng-Lan Wang, Chuan-
Min Tao, Hong Tang 作者: StephenW 时间: 2016-10-11 20:18