1Department of Surgery, the Icahn School of Medicine at Mount Sinai, New York, NY.
2Department of Pathology, the Icahn School of Medicine at Mount Sinai, New York, NY.
AbstractSTATEMENTS OF THE PROBLEM: Quantitation of hepatitis B surface antigen (HBsAg) in hepatitis B-associated hepatocellular carcinoma (HBV-HCC) remains to be clearly defined. This study aims to determine the association of HBsAg quantity with intrahepatic HBV viral load and activity in both tumor and non-neoplastic liver of HBV-HCC patients.
METHOD OF STUDY: Data were obtained from 89 prospectively enrolled patients treated with primary liver resection for HBV-HCC at a single Western institution (2008-2013). Circulating HBsAg was quantitated using ELISA. HBV DNA, covalently closed circular (cccDNA) and precore-pregenomic RNA (preC-pgRNA) in both tumor and non-neoplastic liver were quantitated by real-time PCR from fresh liver resection specimens.
RESULTS: Circulating HBsAg was detectable in all 89 patients. HBsAg negatively correlated with age, and positively correlated with pre-operative serum AFP and ALT levels. HBsAg correlated with HBV cccDNA copy number in tumor or non-neoplastic liver tissue. It also correlated with preC-pgRNA copy number in non-neoplastic liver tissue. HBsAg did not correlate with serum HBV DNA, total intrahepatic HBV DNA, viral replicative activity or transcriptional activity.
CONCLUSIONS: In HBV-HCC patients, HBsAg levels correlated with cccDNA copy number in tumor or non-neoplastic liver tissue, suggesting that a greater pool of cccDNA is associated with a higher rate of HBsAg production. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
"suggesting that a greater pool of cccDNA is associated with a higher rate of HBsAg production. "
我们现在测的表面抗原数字是不是就是rate of HBsAg production？应该是的！
可是这个数字在抗病毒情况下还是变化的？作者: 中国先生ws 时间: 2015-7-17 22:14