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标题: 神秘的乙型肝炎和E患者病因不明的急性肝炎 [打印本页]

作者: StephenW 时间: 2015-7-10 16:39 标题: 神秘的乙型肝炎和E患者病因不明的急性肝炎

Cryptic Hepatitis B and E in Patients With Acute Hepatitis of Unknown Etiology

- Author Affiliations

1Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia
2Vabiotech, Hanoi, Vietnam
3Cornwall Gastrointestinal Unit, Royal Cornwall Hospital Trust and European Centre for the Environment and Human Health, University of Exeter Medical School, Truro, United Kingdom

Correspondence: Lilia Ganova-Raeva, PhD, 1600 Clifton Rd NE, MS A-33, Atlanta, GA 30329 ([email protected]).

Abstract

Background. Up to 30% of acute viral hepatitis has no known etiology. To determine the disease etiology in patients with acute hepatitis of unknown etiology (HUE), serum specimens were obtained from 38 patients residing in the United Kingdom and Vietnam and from 26 healthy US blood donors. All specimens tested negative for known viral infections causing hepatitis, using commercially available serological and nucleic acid assays.

Methods. Specimens were processed by sequence-independent complementary DNA amplification and next-generation sequencing (NGS). Sufficient material for individual NGS libraries was obtained from 12 HUE cases and 26 blood donors; the remaining HUE cases were sequenced as a pool. Read mapping was done by targeted and de novo assembly.

Results. Sequences from hepatitis B virus (HBV) were detected in 7 individuals with HUE (58.3%) and the pooled library, and hepatitis E virus (HEV) was detected in 2 individuals with HUE (16.7%) and the pooled library. Both HEV-positive cases were coinfected with HBV. HBV sequences belonged to genotypes A, D, or G, and HEV sequences belonged to genotype 3. No known hepatotropic viruses were detected in the tested normal human sera.

Conclusions. NGS-based detection of HBV and HEV infections is more sensitive than using commercially available assays. HBV and HEV may be cryptically associated with HUE.

Key words

Received December 10, 2014.
Accepted May 25, 2015.

Published by Oxford University Press on behalf of the Infectious Diseases Society of America 2015. This work is written by (a) US Government employee(s) and is in the public domain in the US.

作者: StephenW 时间: 2015-7-10 16:44

神秘的乙型肝炎和E患者病因不明的急性肝炎

莉利亚Ganova-Raeva1，丽丽Punkova1，大卫S. Campo1，卓娅Dimitrova1，帕维尔Skums1，雅H. Vu2低，做。 T. DAT2，哈里R. Dalton3和尤里·Khudyakov1

-
作者机构

病毒性肝炎，美国疾病控制和预防，亚特兰大，佐治亚州的1区
2Vabiotech，河内，越南
3Cornwall胃肠部，皇家康沃尔医院的信任和欧洲为中心的环境和人类健康，埃克塞特大学医学院，特鲁罗，英国

函授：莉利亚Ganova-Raeva，博士1600克利夫顿路NE，MS A-33，美国佐治亚州亚特兰大30329（[email protected]）。

抽象

背景。达急性病毒性肝炎30％没有已知的病因。为了确定患者的病因不明（HUE）急性肝炎疾病的病因，血清标本来自居住在英国和越南的26名健康美国献血者38例患者获得。所有标本检查阴性已知病毒感染引起肝炎，使用可商购的血清学和核酸测定。

方法。标本由序列互补的独立DNA扩增和新一代测序（NGS）进行处理。从12 HUE案件和26献血者获得个人NGS库足够的材料;其余案件HUE测序一个游泳池。读出的映射是通过有针对性的和从头组装完成。

结果。在7个体HUE（58.3％）和合并的文库，和戊型肝炎病毒（HEV）在2个个体与HUE（16.7％）和合并的文库中检测到了检测乙肝病毒（HBV）的序列。无论HEV阳性病例合并感染乙肝病毒。乙肝病毒序列属于基因型A，D，或G，和HEV序列属于基因在测试正常人血清中检测到3没有已知嗜肝病毒。

结论。 NGS基于检测乙肝病毒和戊型肝炎病毒感染是比使用市售测定更敏感。乙肝病毒和戊型肝炎病毒可与HUE被含糊有关。
关键词

病因不明的肝病新一代测序散弹枪库肝炎

收到的2014年12月10日。
接受2015年5月25日。

发布时间由牛津大学出版社代表传染病协会2015年美国的这项工作是写的（一）美国政府雇员（S），是在美国的公共领域。

作者: 682256 时间: 2015-7-11 19:11

NGS专业术语，再看看！本文是说，急性乙肝和戊肝发生，可能和未知肝炎感染有关。

作者: 682256 时间: 2015-7-11 19:13

NGS，下一代测序？

作者: StephenW 时间: 2015-7-11 19:31

回复 682256 的帖子

病因不明急性肝炎疾病 - 现在的核酸检测没有发现病因.
但NGS - 下一代DNA测序 - 发现乙肝病毒(58.3%)和戊型肝炎病毒(16.7%).

作者: 9病成医 时间: 2015-7-13 16:02

马克。。。

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