标题: HBV and HCC - 3 abstracts. [打印本页] 作者: StephenW 时间: 2011-12-19 21:50 标题: HBV and HCC - 3 abstracts.
本帖最后由 StephenW 于 2011-12-19 22:01 编辑
World J Gastroenterol. 2011 Nov 28;17(44):4853-7.
Hepatitis B virus infection and the risk of hepatocellular carcinoma.
Tan YJ.
Source: http://www.ncbi.nlm.nih.gov/pubmed/22171125
Ya-Jun Tan, Department of Laboratory Medicine, The First Affiliated
Hospital, College of Medicine, Zhejiang University, Hangzhou 310003,
Zhejiang Province, China.
Abstract
Epidemiological studies have provided overwhelming evidence for a causal
role of chronic hepatitis B virus (HBV) infection in the development of
hepatocellular carcinoma (HCC). However, the pathogenesis of HBV infection
and carcinogenesis of HBV-associated HCC are still elusive. This review
will summarize the current knowledge on the mechanisms involved in
HBV-related liver carcinogenesis. The role of HBV in tumor formation
appears to be complex, and may involve both direct and indirect mechanisms.
Integration of HBV DNA into the host genome occurs at early steps of clonal
tumor expansion, and it has been shown to enhance the host chromosomal
instability, leading to large inverted duplications, deletions and
chromosomal translocations. It has been shown that the rate of chromosomal
alterations is increased significantly in HBV-related tumors. Prolonged
expression of the viral regulatory HBV x protein may contribute to
regulating cellular transcription, protein degradation, proliferation, and
apoptotic signaling pathways, and it plays a critical role in the
development of hepatocellular carcinoma. 作者: StephenW 时间: 2011-12-19 21:50
PLoS One. 2011;6(12):e28798. Epub 2011 Dec 8.
Source: http://www.ncbi.nlm.nih.gov/pubmed/22174901
Genome-wide association study of hepatocellular carcinoma in southern
chinese patients with chronic hepatitis B virus infection.
Chan KY, Wong CM, Kwan JS, Lee JM, Cheung KW, Yuen MF, Lai CL, Poon RT,
Sham PC, Ng IO. SourceState Key Laboratory for Liver Research, The
University of Hong Kong, Hong Kong.
Abstract One of the most relevant risk
factors for hepatocellular carcinoma (HCC) development is chronic hepatitis
B virus (HBV) infection, but only a fraction of chronic HBV carriers
develop HCC, indicating that complex interactions among viral,
environmental and genetic factors lead to HCC in HBV-infected patients. So
far, host genetic factors have incompletely been characterized. Therefore,
we performed a genome-wide association (GWA) study in a Southern Chinese
cohort consisting of 95 HBV-infected HCC patients (cases) and 97
HBV-infected patients without HCC (controls) using the Illumina
Human610-Quad BeadChips. The top single nucleotide polymorphisms (SNPs)
were then validated in an independent cohort of 500 cases and 728 controls.
4 SNPs (rs12682266, rs7821974, rs2275959, rs1573266) at chromosome 8p12
showed consistent association in both the GWA and replication phases
(OR(combined)?=?1.31-1.39; p(combined)?=?2.71×10(-5)-5.19×10(-4);
PAR(combined)?=?26-31%). We found a 2.3-kb expressed sequence tag (EST) in
the region using in-silico data mining and verified the existence of the
full-length EST experimentally. The expression level of the EST was
significantly reduced in human HCC tumors in comparison to the
corresponding non-tumorous liver tissues (P<0.001). Results from sequence
analysis and in-vitro protein translation study suggest that the transcript
might function as a long non-coding RNA. In summary, our study suggests
that variations at chromosome 8p12 may promote HCC in patients with HBV.
Further functional studies of this region may help understand
HBV-associated hepatocarcinogenesis.作者: StephenW 时间: 2011-12-19 21:56
PLoS One. 2011;6(12):e28486. Epub 2011 Dec 8.
Source: http://www.ncbi.nlm.nih.gov/pubmed/22174818
Serum MicroRNAs as Biomarkers for Hepatocellular Carcinoma in Chinese
Patients with Chronic Hepatitis B Virus Infection.
Qi P, Cheng SQ, Wang H,
Li N, Chen YF, Gao CF. SourceDepartment of Laboratory Medicine, Second
Military Medical University, Eastern Hepatobiliary Hospital, Shanghai,
China.
Abstract
BACKGROUND: MicroRNAs (miRNAs) have been shown to anticipate great cancer
diagnostic potential. Recently, circulating miRNAs have been reported as
promising biomarkers for various pathologic conditions. The objective of
this study was to investigate the potential of serum miRNAs as novel
biomarkers for hepatocellular carcinoma (HCC).
METHODOLOGY/PRINCIPAL FINDINGS: THIS STUDY WAS DIVIDED INTO FOUR PHASES:
(I) Ten candidate serum miRNAs were detected by using real-time RT-PCR,
corresponding 10 HCC patients with chronic hepatitis B virus (HBV)
infection and 10 age- and sex-matched healthy subjects. (II) Marker
validation by real-time RT-PCR on HBV patients with (n?=?48) or without HCC
(n?=?48), and healthy subjects (n?=?24). (III) Marker detection by
real-time RT-PCR in sera from another 14 HCC patients before and 1 month
after surgical resection. (IV) We examined the correlation between the
expressions of candidate serum miRNAs with clinical parameters of HCC
patients. Although miR-222, miR-223 or miR-21 were significantly up- or
down-regulated between HCC patients and healthy controls, no significant
difference was observed in the levels of these miRNAs between HBV patients
without and with HCC. MiR-122 in serum was significantly higher in HCC
patients than healthy controls (p<0.001). More importantly, it was found
that the levels of miR-122 were significantly reduced in the post-operative
serum samples when compared to the pre-operative samples. Although serum
miR-122 was also elevated in HBV patients with HCC comparing with those
without HCC, the difference was at the border line (p?=?0.043).
CONCLUSIONS/SIGNIFICANCE: Our results suggest that serum miR-122 might
serve as a novel and potential noninvasive biomarker for detection of HCC
in healthy subjects, moreover, it might serve as a novel biomarker for
liver injury but not specifically for detection of HCC in chronic HBV
infection patients. 作者: StephenW 时间: 2011-12-19 21:59